Any feedback?
Literature summary extracted from
- D-Arabinose (L-fucose) isomerase from Aerobacter aerogenes (1975), Methods Enzymol., 41B, 462-465.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.3.1.3 | - |
Klebsiella pneumoniae |
| 5.3.1.25 | - |
Klebsiella pneumoniae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.3 | 2-amino-2-methyl-1,3-propanediol | - |
Klebsiella pneumoniae |  |
| 5.3.1.3 | D-arabitol | - |
Klebsiella pneumoniae | |
| 5.3.1.3 | ribitol | - |
Klebsiella pneumoniae | |
| 5.3.1.3 | Tris | - |
Klebsiella pneumoniae | |
| 5.3.1.3 | xylitol | - |
Klebsiella pneumoniae | |
| 5.3.1.25 | 2-amino-2-methyl-1,3-propanediol | - |
Klebsiella pneumoniae | |
| 5.3.1.25 | D-arabitol | - |
Klebsiella pneumoniae | |
| 5.3.1.25 | ribitol | - |
Klebsiella pneumoniae | |
| 5.3.1.25 | Tris | - |
Klebsiella pneumoniae | |
| 5.3.1.25 | xylitol | - |
Klebsiella pneumoniae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.3 | 51 | - |
L-fucose | - |
Klebsiella pneumoniae | |
| 5.3.1.3 | 160 | - |
D-arabinose | D-arabinose | Klebsiella pneumoniae | |
| 5.3.1.25 | 51 | - |
L-fucose | - |
Klebsiella pneumoniae | |
| 5.3.1.25 | 160 | - |
D-arabinose | D-arabinose | Klebsiella pneumoniae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.3 | Mn2+ | required | Klebsiella pneumoniae | |
| 5.3.1.25 | Mn2+ | required | Klebsiella pneumoniae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.3 | 250000 | - |
gel filtration | Klebsiella pneumoniae |
| 5.3.1.25 | 250000 | - |
gel filtration | Klebsiella pneumoniae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.3 | Klebsiella pneumoniae | - |
- |
- |
| 5.3.1.3 | Klebsiella pneumoniae M-7 | - |
- |
- |
| 5.3.1.25 | Klebsiella pneumoniae | - |
- |
- |
| 5.3.1.25 | Klebsiella pneumoniae M-7 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.3 | - |
Klebsiella pneumoniae |
| 5.3.1.25 | - |
Klebsiella pneumoniae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.3 | 6 | - |
- |
Klebsiella pneumoniae |
| 5.3.1.25 | 6 | - |
- |
Klebsiella pneumoniae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.3.1.3 | 2°C, crystalline enzyme is stable as a sediment in polyethylene glycol solution for at least 1 month | Klebsiella pneumoniae |
| 5.3.1.3 | stable for more than 1 month in 50 mM Tris-HCl buffer at pH 7.5 containing 1 mM MnCl2 and mercaptoethanol | Klebsiella pneumoniae |
| 5.3.1.25 | 2°C, crystalline enzyme is stable as a sediment in polyethylene glycol solution for at least 1 month | Klebsiella pneumoniae |
| 5.3.1.25 | stable for more than 1 month in 50 mM Tris-HCl buffer at pH 7.5 containing 1 mM MnCl2 and mercaptoethanol | Klebsiella pneumoniae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.3 | 8 | 10 | - |
Klebsiella pneumoniae |
| 5.3.1.25 | 8 | 10 | - |
Klebsiella pneumoniae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
