EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
5.2.1.2 | GSH | required as coenzyme | Vibrio sp. | |
5.2.1.2 | GSH | other thiols are inactive | Vibrio sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.2.1.2 | GSSG | - |
Vibrio sp. | |
5.2.1.2 | N-Hexylmaleamic acid | noncompetitive towards GSH and maleylacetone | Vibrio sp. | |
5.2.1.2 | NEM | - |
Vibrio sp. | |
5.2.1.2 | S-Glutathioyl-N-hexylmaleamate | competitive towards GSH, uncompetitive towards maleylacetone | Vibrio sp. | |
5.2.1.2 | S-Methylglutathione | - |
Vibrio sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.2.1.2 | 0.8 | - |
maleylacetone | - |
Vibrio sp. | |
5.2.1.2 | 1.9 | - |
6-Oxo-2(E),4(Z)-heptanedienoic acid | - |
Vibrio sp. | |
5.2.1.2 | 2.9 | - |
trans-beta-Acetylacrylate | - |
Vibrio sp. | |
5.2.1.2 | 3 | - |
cis-beta-Acetylacrylate | - |
Vibrio sp. | |
5.2.1.2 | 3.2 | - |
6-Oxo-2(Z),4(E)-heptanedienoic acid | - |
Vibrio sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.2.1.2 | 16000 | - |
x * 16000, SDS-PAGE after heating the enzyme at 100°C for 10 min in a standard aqueous medium containing 1% SDS and 8M urea | Vibrio sp. |
5.2.1.2 | 35000 | - |
gel filtration | Vibrio sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.2.1.2 | Maleylacetoacetate | Vibrio sp. | one of the enzymes responsible for the oxidative metabolism of aromatic amino acids | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.2.1.2 | Mammalia | - |
- |
- |
5.2.1.2 | Vibrio sp. | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
5.2.1.2 | liver | - |
Mammalia | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.2.1.2 | 6-Oxo-2(E),4(Z)-heptanedienoic acid | - |
Vibrio sp. | 6-Oxo-2(Z),4(E)-heptanedienoic acid | - |
? | |
5.2.1.2 | cis-beta-Acetylacrylate | - |
Vibrio sp. | trans-beta-Acetylacrylate | - |
? | |
5.2.1.2 | Maleylacetoacetate | - |
Vibrio sp. | Fumarylacetoacetate | - |
? | |
5.2.1.2 | Maleylacetoacetate | one of the enzymes responsible for the oxidative metabolism of aromatic amino acids | Vibrio sp. | ? | - |
? | |
5.2.1.2 | maleylacetone | - |
Vibrio sp. | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.2.1.2 | ? | x * 35000, SDS-PAGE | Vibrio sp. |
5.2.1.2 | ? | x * 16000, SDS-PAGE after heating the enzyme at 100°C for 10 min in a standard aqueous medium containing 1% SDS and 8M urea | Vibrio sp. |