Any feedback?
Literature summary extracted from
- Overview: the Na,K-pump (1988), Methods Enzymol., 156, 1-25.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.2.13 | oligomycin | not completely inhibits but decreases the rate of turnover of transport and of the hydrolysis reaction. Oligomycin affects the extracellular side of the system, Na+ on the cytoplasmic side of the system is necessary for the effect, oligomycin decreases the rate of transition from the Na+ form to the K+ form of the enzyme, but has no effect on the rate of the reverse reaction | Mammalia |  |
| 7.2.2.13 | oubain | K+ protects against inhibition, probably due to phosphorylating effect | Mammalia | |
| 7.2.2.13 | vanadate | - |
Mammalia | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.2.2.13 | membrane | - |
Mammalia | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.2.13 | Cs+ | the Na,K pump is activated by the combined effect of Na+ on the cytoplasmic side and of K+ on the extracellular side of the membrane. K+ can be replaced by the following monovalent cations in the order of decreasing activation: K+, Rb+, NH4+, Cs+, Li, Na+ | Mammalia | |
| 7.2.2.13 | K+ | the Na,K pump is activated by the combined effect of Na+ on the cytoplasmic side and of K+ on the extracellular side of the membrane. K+ can be replaced by the following monovalent cations in the order of decreasing activation: Rb+, NH4+, Cs+, Li, Na+ | Mammalia | |
| 7.2.2.13 | Li+ | the Na,K pump is activated by the combined effect of Na+ on the cytoplasmic side and of K+ on the extracellular side of the membrane. K+ can be replaced by the following monovalent cations in the order of decreasing activation: K+, Rb+, NH4+, Cs+, Li, Na+ | Mammalia | |
| 7.2.2.13 | Na+ | the Na,K pump is activated by the combined effect of Na+ on the cytoplasmic side and of K+ on the extracellular side of the membrane. Na+ cannot be replaced by any other monovalent cation. K+ can be replaced by the following monovalent cations in the order of decreasing activation: K+, Rb+, NH4+, Cs+, Li, Na+ | Mammalia | |
| 7.2.2.13 | NH4+ | the Na,K pump is activated by the combined effect of Na+ on the cytoplasmic side and of K+ on the extracellular side of the membrane. K+ can be replaced by the following monovalent cations in the order of decreasing activation: K+, Rb+, NH4+, Cs+, Li, Na+ | Mammalia | |
| 7.2.2.13 | Rb+ | the Na,K pump is activated by the combined effect of Na+ on the cytoplasmic side and of K+ on the extracellular side of the membrane. K+ can be replaced by the following monovalent cations in the order of decreasing activation: K+, Rb+, NH4+, Cs+, Li, Na+ | Mammalia | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.2.2.13 | 38000 | - |
x * 100000, alpha-subunit, + x * 38000, beta-subunit, the ratio of alpha: beta is 1:1 | Mammalia |
| 7.2.2.13 | 100000 | - |
x * 100000, alpha-subunit, + x * 38000, beta-subunit, the ratio of alpha: beta is 1:1 | Mammalia |
| 7.2.2.13 | 379000 | - |
equilibrium sedimentation | shark |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.2.13 | ATP + H2O + Na+/in + K+/out | Mammalia | creation of a electrochemical gradient of Na+ ions into and K+ ions out of the cell | ADP + phosphate + Na+/out + K+/in | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.2.2.13 | Mammalia | - |
- |
- |
| 7.2.2.13 | shark | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 7.2.2.13 | glycoprotein | the beta subunit is a glycoprotein | Mammalia |
| 7.2.2.13 | side-chain modification | required for activity, removal of lipids leads to inactivation and readdition of the lipids results in reactivation of the enzyme | Mammalia |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.2.2.13 | - |
shark |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.2.13 | ATP + H2O + Na+/in + K+/out | - |
shark | ADP + phosphate + Na+/out + K+/in | - |
? | |
| 7.2.2.13 | ATP + H2O + Na+/in + K+/out | for each ATP molecule hydrolyzed three Na+ ions are transported out of and two K+ ions into the cell | Mammalia | ADP + phosphate + Na+/out + K+/in | - |
? | |
| 7.2.2.13 | ATP + H2O + Na+/in + K+/out | creation of a electrochemical gradient of Na+ ions into and K+ ions out of the cell | Mammalia | ADP + phosphate + Na+/out + K+/in | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.2.2.13 | ? | x * 100000, alpha-subunit, + x * 38000, beta-subunit, the ratio of alpha: beta is 1:1 | Mammalia |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
