BRENDA - Enzyme Database show

Nucleotide pyrophosphatase

Kornberg, A.; Pricer, W.E.; J. Biol. Chem. 182, 763-778 (1950)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.9
5'-AMP
49% inhibition at 40 mM
Solanum tuberosum
3.6.1.9
Borate
strong inhibition at pH 8.5, not at pH 7.4
Solanum tuberosum
3.6.1.9
diphosphate
strong inhibition at pH 8.5, not at pH 7.4
Solanum tuberosum
3.6.1.9
NADH
effective inhibition of DPN splitting
Solanum tuberosum
3.6.1.9
nicotinamide
19% inhibition at 100 mM
Solanum tuberosum
3.6.1.9
NMN
10% inhibition at 40 mM
Solanum tuberosum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.9
ATP + H2O
Solanum tuberosum
-
AMP + diphosphate
-
Solanum tuberosum
?
3.6.1.9
FAD + H2O
Solanum tuberosum
-
FMN + AMP
-
Solanum tuberosum
?
3.6.1.9
NADH + H2O
Solanum tuberosum
reduced and oxidized forms
NMNH + AMP
-
Solanum tuberosum
?
3.6.1.9
NADP+ + H2O
Solanum tuberosum
-
nicotinamide nucleotide + adenosine 2',5'-bisphosphate
-
Solanum tuberosum
?
3.6.1.9
thiamine diphosphate + H2O
Solanum tuberosum
-
?
-
Solanum tuberosum
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.9
Solanum tuberosum
-
potato
-
Purification (Commentary)
EC Number
Commentary
Organism
3.6.1.9
-
Solanum tuberosum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.6.1.9
tuber
-
Solanum tuberosum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.9
36.7
-
-
Solanum tuberosum
Storage Stability
EC Number
Storage Stability
Organism
3.6.1.9
0-5°C, remarkably stable within 15 months
Solanum tuberosum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.9
ATP + H2O
-
246834
Solanum tuberosum
AMP + diphosphate
-
246834
Solanum tuberosum
?
3.6.1.9
FAD + H2O
-
246834
Solanum tuberosum
FMN + AMP
-
246834
Solanum tuberosum
?
3.6.1.9
NADH + H2O
reduced and oxidized forms
246834
Solanum tuberosum
NMNH + AMP
-
246834
Solanum tuberosum
?
3.6.1.9
NADP+ + H2O
-
246834
Solanum tuberosum
nicotinamide nucleotide + adenosine 2',5'-bisphosphate
-
246834
Solanum tuberosum
?
3.6.1.9
thiamine diphosphate + H2O
-
246834
Solanum tuberosum
?
-
246834
Solanum tuberosum
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.9
6.5
8.5
-
Solanum tuberosum
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.6.1.9
4
-
50% decreased activity
Solanum tuberosum
3.6.1.9
9
-
50% decreased activity
Solanum tuberosum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.9
5'-AMP
49% inhibition at 40 mM
Solanum tuberosum
3.6.1.9
Borate
strong inhibition at pH 8.5, not at pH 7.4
Solanum tuberosum
3.6.1.9
diphosphate
strong inhibition at pH 8.5, not at pH 7.4
Solanum tuberosum
3.6.1.9
NADH
effective inhibition of DPN splitting
Solanum tuberosum
3.6.1.9
nicotinamide
19% inhibition at 100 mM
Solanum tuberosum
3.6.1.9
NMN
10% inhibition at 40 mM
Solanum tuberosum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.9
ATP + H2O
Solanum tuberosum
-
AMP + diphosphate
-
Solanum tuberosum
?
3.6.1.9
FAD + H2O
Solanum tuberosum
-
FMN + AMP
-
Solanum tuberosum
?
3.6.1.9
NADH + H2O
Solanum tuberosum
reduced and oxidized forms
NMNH + AMP
-
Solanum tuberosum
?
3.6.1.9
NADP+ + H2O
Solanum tuberosum
-
nicotinamide nucleotide + adenosine 2',5'-bisphosphate
-
Solanum tuberosum
?
3.6.1.9
thiamine diphosphate + H2O
Solanum tuberosum
-
?
-
Solanum tuberosum
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.9
-
Solanum tuberosum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.6.1.9
tuber
-
Solanum tuberosum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.9
36.7
-
-
Solanum tuberosum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.6.1.9
0-5°C, remarkably stable within 15 months
Solanum tuberosum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.9
ATP + H2O
-
246834
Solanum tuberosum
AMP + diphosphate
-
246834
Solanum tuberosum
?
3.6.1.9
FAD + H2O
-
246834
Solanum tuberosum
FMN + AMP
-
246834
Solanum tuberosum
?
3.6.1.9
NADH + H2O
reduced and oxidized forms
246834
Solanum tuberosum
NMNH + AMP
-
246834
Solanum tuberosum
?
3.6.1.9
NADP+ + H2O
-
246834
Solanum tuberosum
nicotinamide nucleotide + adenosine 2',5'-bisphosphate
-
246834
Solanum tuberosum
?
3.6.1.9
thiamine diphosphate + H2O
-
246834
Solanum tuberosum
?
-
246834
Solanum tuberosum
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.9
6.5
8.5
-
Solanum tuberosum
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.6.1.9
4
-
50% decreased activity
Solanum tuberosum
3.6.1.9
9
-
50% decreased activity
Solanum tuberosum