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Literature summary extracted from

  • Bischof, E.; Tran-Thi, T.A.; Decker, K.F.A.
    Nucleotide pyrophosphatase of rat liver. A comparative study on the enzymes solubilized and purified from plasma membrane and endoplasmic reticulum (1975), Eur. J. Biochem., 51, 353-361.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.9 AMP(CH)2PP competitive inhibition, Ki: 25 mM Rattus sp.
3.6.1.9 AMP-amidate competitive inhibition, Ki: 30 mM Rattus sp.
3.6.1.9 AMP-PP(S) competitive inhibition, Ki: 33 mM Rattus sp.
3.6.1.9 EDTA complete inactivation, partially restored by Mg2+, Ca2+ Rattus sp.
3.6.1.9 UMP competitive inhibition, Ki: 26 mM Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.9 15
-
 thymidine 5'-monophosphate p-nitrophenyl ester
-
 Rattus sp.
3.6.1.9 20
-
 NADH
-
 Rattus sp.
3.6.1.9 24
-
 UDP-glucose
-
 Rattus sp.
3.6.1.9 28
-
 ATP
-
 Rattus sp.
3.6.1.9 28
-
 NAD+
-
 Rattus sp.
3.6.1.9 30
-
 UTP
-
 Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.9 endoplasmic reticulum
-
 Rattus sp. 5783
-
3.6.1.9 nucleus
-
 Rattus sp. 5634
-
3.6.1.9 plasma membrane
-
 Rattus sp. 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.9 Ca2+ required for optimal activity Rattus sp.
3.6.1.9 Mg2+ required for optimal activity Rattus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.9 137000
-
 1 * 137000, SDS-PAGE Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.9 ATP + H2O Rattus sp.
-
 AMP + diphosphate
-
 ?
3.6.1.9 NAD+ + H2O Rattus sp.
-
 NMN + AMP
-
 ?
3.6.1.9 NADH + H2O Rattus sp.
-
 NMNH + AMP
-
 ?
3.6.1.9 UDP-glucose + H2O Rattus sp.
-
 UMP + D-glucose-1-phosphate
-
 ?
3.6.1.9 UTP + H2O Rattus sp.
-
 UMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.9 Rattus sp.
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.6.1.9 glycoprotein
-
 Rattus sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.9 to homogeneity, affinity chromatography Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.9 liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.9 145 182
-
 Rattus sp.

Storage Stability

EC Number Storage Stability Organism
3.6.1.9 4°C, lyophilized, no loss of activity Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.9 ATP + H2O
-
 Rattus sp. AMP + diphosphate
-
 ?
3.6.1.9 bis(p-nitrophenyl)phosphate + H2O
-
 Rattus sp. p-nitrophenol + p-nitrophenylphosphate
-
 ?
3.6.1.9 NAD+ + H2O
-
 Rattus sp. NMN + AMP
-
 ?
3.6.1.9 NADH + H2O
-
 Rattus sp. NMNH + AMP
-
 ?
3.6.1.9 thymidine 5'-monophosphate p-nitrophenyl ester + H2O
-
 Rattus sp. thymidine 5'-monophosphate + p-nitrophenol
-
 ?
3.6.1.9 UDP-glucose + H2O
-
 Rattus sp. UMP + D-glucose-1-phosphate
-
 ?
3.6.1.9 UTP + H2O
-
 Rattus sp. UMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.9 monomer 1 * 137000, SDS-PAGE Rattus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.9 9.7
-
-
 Rattus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.6.1.9 6
-
 inactivation below, very stable at alkaline pH Rattus sp.