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Literature summary extracted from

  • Murata, K.
    Action of two types of thiaminase on thiamine and its analogues (1982), Ann. N. Y. Acad. Sci., 378, 146-156.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.99.2 0.003
-
 thiamine
-
 Aneurinibacillus aneurinilyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.99.2 100000
-
 gel filtration Aneurinibacillus aneurinilyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.99.2 thiamine + H2O Aneurinibacillus aneurinilyticus
-
 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.2 Aneurinibacillus aneurinilyticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.99.2 2-thiothiamine + H2O
-
 Aneurinibacillus aneurinilyticus ?
-
 ?
3.5.99.2 thiamine + H2O
-
 Aneurinibacillus aneurinilyticus 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.99.2 37
-
-
 Aneurinibacillus aneurinilyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.99.2 8.6
-
-
 Aneurinibacillus aneurinilyticus