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Literature summary extracted from

  • te Boemmelstroet, B.W.; Hensgens, C.M.H.; Geerts, W.J.; Keltjens, J.T.; van der Drift, C.; Vogels, G.D.
    Purification and properties of 5,10-methenyltetrahydromethanopterin cyclohydrolase from Methanosarcina barkeri (1990), J. Bacteriol., 172, 564-571.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.5.4.27 detergents are essential for stability Methanosarcina barkeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.27 0.57
-
 N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin
-
 Methanosarcina barkeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.27 Mg2+ 9-18 mM: no stimulation Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.27 41000
-
 2 * 41000, SDS-PAGE Methanosarcina barkeri
3.5.4.27 82000
-
 non-denaturing PAGE Methanosarcina barkeri

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.27 Methanosarcina barkeri
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.5.4.27 stable to oxygen Methanosarcina barkeri
3.5.4.27 the purified enzyme is more resistant to inactivation by O2 than the enzyme in cell extract Methanosarcina barkeri

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.27
-
 Methanosarcina barkeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.27 470
-
-
 Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methanosarcina barkeri N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 r

Subunits

EC Number Subunits Comment Organism
3.5.4.27 dimer 2 * 41000, SDS-PAGE Methanosarcina barkeri