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Literature summary extracted from
- N1-(5'-phosphoribosyl)adenosine-5'-monophosphate cyclohydrolase: purification and characterization of a unique metalloenzyme (1999), Biochemistry, 38, 1537-1546.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.4.19 | overexpression in Escherichia coli | Methanococcus vannielii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.19 | EDTA | reversible inhibition | Methanococcus vannielii |  |
| 3.5.4.19 | Zn2+ | exogenous Zn2+ | Methanococcus vannielii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.4.19 | 0.009 | - |
1-(5-phosphoribosyl)-AMP | - |
Methanococcus vannielii | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.19 | Zn2+ | a single tightly bound Zn2+ per subunit | Methanococcus vannielii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.19 | 15486 | - |
alpha2, 2 * 15486, MALDI-MS | Methanococcus vannielii |
| 3.5.4.19 | 31200 | - |
ultracentrifugation | Methanococcus vannielii |
| 3.5.4.19 | 34000 | - |
gel filtration | Methanococcus vannielii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.19 | 1-(5-phosphoribosyl)-AMP + H2O | Methanococcus vannielii | - |
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.19 | Methanococcus vannielii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.4.19 | - |
Methanococcus vannielii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.19 | 17.6 | - |
- |
Methanococcus vannielii |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.5.4.19 | -20°C, pellet by polyethylene glycol and MgCl2, stable till used | Methanococcus vannielii |
| 3.5.4.19 | 4°C, phosphate buffer, pH 7.5, one month | Methanococcus vannielii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.19 | 1-(5-phosphoribosyl)-AMP + H2O | - |
Methanococcus vannielii | 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.4.19 | dimer | alpha2, 2 * 15486, MALDI-MS | Methanococcus vannielii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.19 | 30 | - |
assays at 30°C | Methanococcus vannielii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.19 | 7.5 | - |
- |
Methanococcus vannielii |
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