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Literature summary extracted from

  • Weidanz, J.A.; Campbell, P.; Moore, D.; deLucas, L.J.; Roden, L.; Thompson, J.N.; Vezza, P.
    N-Acetylglucosamine kinase and N-acetylglucosamine 6-phosphate deacetylase in normal human erythrocytes and Plasmodium falciparum (1996), Br. J. Haematol., 95, 645-653.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.59 N-acetyl-D-glucosamine 6-phosphate
-
 Homo sapiens
2.7.1.59 N-acetyl-D-glucosamine 6-phosphate
-
 Plasmodium falciparum
3.5.1.25 EDTA some reversal by MgCl2, CoCl2 restores activity and stimulates above the control value Homo sapiens
3.5.1.25 fructose 1,6-diphosphate 72% inhibition at 0.2 mM and 90% inhibition at 0.7 mM Homo sapiens
3.5.1.25 fructose-6-phosphate 20% inhibition at 0.3 mM, 30% inhibition at 0.9 mM Homo sapiens
3.5.1.25 glucosamine 6-phosphate 87% inhibition at 0.4 mM, 95% inhibition at 1.2 mM Homo sapiens
3.5.1.25 glucose 6-phosphate 55% inhibition at 0.35 mM, 80% inhibition at 1.1 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.59 0.089
-
 N-acetyl-D-glucosamine 37°C, pH 7.5 Homo sapiens
3.5.1.25 0.029
-
 N-acetylglucosamine 6-phosphate
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.59 Mg2+ required Homo sapiens
2.7.1.59 Mg2+ required Plasmodium falciparum
3.5.1.25 Co2+ restores activity after treatment with EDTA and stimulates above the control value Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.59 ATP + N-acetyl-D-glucosamine Homo sapiens
-
 ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.7.1.59 ATP + N-acetyl-D-glucosamine Plasmodium falciparum
-
 ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
3.5.1.25 N-acetyl-D-glucosamine 6-phosphate + H2O Homo sapiens pathway of N-acetylglucosamine metabolism D-glucosamine 6-phosphate + acetate
-
 ?
3.5.1.25 N-acetyl-D-glucosamine 6-phosphate + H2O Plasmodium falciparum pathway of N-acetylglucosamine metabolism D-glucosamine 6-phosphate + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.59 Homo sapiens
-
-
-
2.7.1.59 Plasmodium falciparum
-
 malaria parasite
-
3.5.1.25 Homo sapiens
-
-
-
3.5.1.25 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.25
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.59 erythrocyte
-
 Homo sapiens
-
3.5.1.25 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.59 0.00009
-
 37°C, pH 7.5 Homo sapiens
2.7.1.59 0.00012
-
 37°C, pH 7.5 Plasmodium falciparum
3.5.1.25 additional information
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.5.1.25 4°C, 10 mM Tris-HCl, pH 7.5, 25% glycerol, 2 mM CHAPS, stable for at least 2 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.59 ATP + N-acetyl-D-glucosamine
-
 Homo sapiens ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.7.1.59 ATP + N-acetyl-D-glucosamine
-
 Plasmodium falciparum ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
3.5.1.25 N-acetyl-D-glucosamine 6-phosphate + H2O
-
 Homo sapiens D-glucosamine 6-phosphate + acetate
-
 ?
3.5.1.25 N-acetyl-D-glucosamine 6-phosphate + H2O
-
 Plasmodium falciparum D-glucosamine 6-phosphate + acetate
-
 ?
3.5.1.25 N-acetyl-D-glucosamine 6-phosphate + H2O pathway of N-acetylglucosamine metabolism Homo sapiens D-glucosamine 6-phosphate + acetate
-
 ?
3.5.1.25 N-acetyl-D-glucosamine 6-phosphate + H2O pathway of N-acetylglucosamine metabolism Plasmodium falciparum D-glucosamine 6-phosphate + acetate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.25 7.5
-
-
 Homo sapiens