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Literature summary extracted from

  • Yamano, N.; Matsushita, Y.; Kamada, K.; Fujishima, S.; Arita, M.
    Purification and characterization of N-acetylglucosamine 6-phosphate deacetylase with activity against N-acetylglucosamine from Vibrio cholera Non-O1 (1996), Biosci. Biotechnol. Biochem., 60, 1320-1323.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.25 Cu2+ 1 mM, complete inhibition Vibrio cholerae serotype O1
3.5.1.25 EDTA 1 mM, 25% inhibition Vibrio cholerae serotype O1
3.5.1.25 NEM 1 mM, complete inhibition Vibrio cholerae serotype O1
3.5.1.25 PCMB 0.1 mM, complete inhibition Vibrio cholerae serotype O1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.25 0.03
-
 N-acetylglucosamine 6-phosphate
-
 Vibrio cholerae serotype O1
3.5.1.25 4.6
-
 N-acetylglucosamine 6-sulfate
-
 Vibrio cholerae serotype O1
3.5.1.25 73
-
 N-acetylglucosamine
-
 Vibrio cholerae serotype O1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.25 Co2+ 200% activation at 4 mM Vibrio cholerae serotype O1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.25 45000
-
 4 * 45000, SDS-PAGE Vibrio cholerae serotype O1
3.5.1.25 190000
-
 gel filtration Vibrio cholerae serotype O1

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.25 Vibrio cholerae serotype O1
-
 Non-O1
-
3.5.1.25 Vibrio cholerae serotype O1 Non-O1
-
 Non-O1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.25
-
 Vibrio cholerae serotype O1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.25 25.8
-
-
 Vibrio cholerae serotype O1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.25 N-acetyl-D-glucosamine 6-phosphate + H2O
-
 Vibrio cholerae serotype O1 D-glucosamine 6-phosphate + acetate
-
 ?
3.5.1.25 N-acetyl-D-glucosamine 6-phosphate + H2O
-
 Vibrio cholerae serotype O1 Non-O1 D-glucosamine 6-phosphate + acetate
-
 ?
3.5.1.25 N-acetyl-D-glucosamine 6-sulfate + H2O
-
 Vibrio cholerae serotype O1 D-glucosamine 6-sulfate + acetate
-
 ?
3.5.1.25 N-acetyl-D-glucosamine 6-sulfate + H2O
-
 Vibrio cholerae serotype O1 Non-O1 D-glucosamine 6-sulfate + acetate
-
 ?
3.5.1.25 N-acetylglucosamine + H2O
-
 Vibrio cholerae serotype O1 D-glucosamine + acetate
-
 ?
3.5.1.25 N-acetylglucosamine + H2O
-
 Vibrio cholerae serotype O1 Non-O1 D-glucosamine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.25 tetramer 4 * 45000, SDS-PAGE Vibrio cholerae serotype O1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.25 37
-
-
 Vibrio cholerae serotype O1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.25 29
-
 pH 6.0-8.0, 30 min, stable up to Vibrio cholerae serotype O1
3.5.1.25 30
-
 pH 6.0-8.0, 30 min, enzyme retains more than 70% of its activity Vibrio cholerae serotype O1
3.5.1.25 45
-
 pH 6.0-8.0, 30 min, complete loss of activity Vibrio cholerae serotype O1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.25 0.97
-
 N-acetylglucosamine 6-phosphate
-
 Vibrio cholerae serotype O1
3.5.1.25 25
-
 N-acetylglucosamine
-
 Vibrio cholerae serotype O1
3.5.1.25 50
-
 N-acetylglucosamine 6-sulfate
-
 Vibrio cholerae serotype O1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.25 7.8 8.5
-
 Vibrio cholerae serotype O1

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.25 6 8 30 min, stable up to 29°C, enzyme retains more than 70% of its activity at 30°C, complete loss of activity at 45°C Vibrio cholerae serotype O1