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Purification, gene cloning, targeted knockout, overexpression, and biochemical characterization of the major pyrazinamidase from Mycobacterium smegmatis

Boshoff, H.I.; Mizrahi, V.; J. Bacteriol. 180, 5809-5814 (1998)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.1.19
expression in Escherichia coli
Mycobacterium tuberculosis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.033
-
nicotinamide
-
Mycobacterium tuberculosis
3.5.1.19
0.3
-
Pyrazinamide
-
Mycobacterium tuberculosis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Mycobacterium tuberculosis
-
-
-
3.5.1.19
Mycolicibacterium smegmatis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.19
-
Mycolicibacterium smegmatis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246585
Mycolicibacterium smegmatis
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
246585
Mycobacterium tuberculosis
nicotinate + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
246585
Mycolicibacterium smegmatis
pyrazinoic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
246585
Mycobacterium tuberculosis
pyrazinoic acid + NH3
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
expression in Escherichia coli
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.033
-
nicotinamide
-
Mycobacterium tuberculosis
3.5.1.19
0.3
-
Pyrazinamide
-
Mycobacterium tuberculosis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
-
Mycolicibacterium smegmatis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
246585
Mycolicibacterium smegmatis
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
246585
Mycobacterium tuberculosis
nicotinate + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
246585
Mycolicibacterium smegmatis
pyrazinoic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
246585
Mycobacterium tuberculosis
pyrazinoic acid + NH3
-
-
-
?