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Literature summary extracted from

  • Pace, C.N.; Laurents, D.V.; Thomson, J.A.
    pH Dependence of the urea and guanidine hydrochloride denaturation of ribonuclease A and ribonuclease T1 (1990), Biochemistry, 29, 2564-2572.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.6.1.24 Guanidine hydrochloride, pH-dependence of denaturation Aspergillus oryzae
4.6.1.24 Urea, pH-dependence of urea denaturation Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.18 RNA + H2O Bos taurus endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Bos taurus
-
-
-
4.6.1.24 Aspergillus oryzae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.18 cyclic 2',3'-nucleoside monophosphate + H2O second step of hydrolysis is irreversible Bos taurus 3'-phosphomononucleotides
-
 ir
4.6.1.18 RNA + H2O endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA Bos taurus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 RNA + H2O first step of hydrolysis is reversible Bos taurus cyclic 2',3'-nucleoside monophosphate
-
 r
4.6.1.24 RNA + H2O
-
 Aspergillus oryzae Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ?

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.6.1.18 7 9 maximum conformational stability to urea and guanidine hydrochloride denaturation Bos taurus
4.6.1.24 4.5
-
 maximal conformational stability Aspergillus oryzae