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Literature summary extracted from

  • Deupree, J.; Wood, W.A.
    L-Ribulose-5-phosphate 4-epimerase from Aerobacter aerogenes (1975), Methods Enzymol., 41B, 412-419.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.3.4
-
 Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.4 EDTA reactivated by addition of divalent metal ion in decreasing order: Mn2+, Co2+, Ni2+, Ca2+, Zn2+, Mg2+ Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.4 0.1
-
 L-ribulose 5-phosphate
-
 Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.4 Ca2+ divalent metal ion required. Reactivated by addition of divalent metal ion in decreasing order: Mn2+, Co2+, Ni2+, Ca2+, Zn2+, Mg2+ Klebsiella aerogenes
5.1.3.4 Co2+ divalent metal ion required. Reactivated by addition of divalent metal ion in decreasing order: Mn2+, Co2+, Ni2+, Ca2+, Zn2+, Mg2+ Klebsiella aerogenes
5.1.3.4 Mg2+ divalent metal ion required. Reactivated by addition of divalent metal ion in decreasing order: Mn2+, Co2+, Ni2+, Ca2+, Zn2+, Mg2+ Klebsiella aerogenes
5.1.3.4 Mn2+ reactivated after EDTA treatment by addition of divalent metal ion in decreasing order: Mn2+, Co2+, Ni2+, Ca2+, Zn2+, Mg2+ Klebsiella aerogenes
5.1.3.4 Ni2+ divalent metal ion required. Reactivated by addition of divalent metal ion in decreasing order: Mn2+, Co2+, Ni2+, Ca2+, Zn2+, Mg2+ Klebsiella aerogenes
5.1.3.4 Zn2+ divalent metal ion required. Reactivated by addition of divalent metal ion in decreasing order: Mn2+, Co2+, Ni2+, Ca2+, Zn2+, Mg2+ Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.4 114000
-
 high speed equilibrium analysis Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.4 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.4
-
 Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.4 additional information
-
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.4 L-Ribulose 5-phosphate
-
 Klebsiella aerogenes D-Xylulose 5-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.4 8.5 9.5
-
 Klebsiella aerogenes