BRENDA - Enzyme Database

Glutamyl-tRNA synthetases from wheat. Isolation and characterization of three dimeric enzymes

Ratinaud, M.H.; Thomes, J.C.; Julien, R.; Eur. J. Biochem. 135, 471-477 (1983)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.1.1.17
chloroplast
enzyme form GluRSC
Triticum aestivum
9507
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
55000
-
2 * 55000, SDS-PAGE, chloroplastic enzyme
Triticum aestivum
6.1.1.17
79000
-
2 * 79000, SDS-PAGE, enzyme form GluRSP
Triticum aestivum
6.1.1.17
83000
-
2 * 83000, SDS-PAGE, enzyme form GluRSE
Triticum aestivum
6.1.1.17
110000
112000
non-denaturing PAGE, gel filtration, chloroplastic enzyme
Triticum aestivum
6.1.1.17
155000
160000
gel filtration, non-denaturing PAGE, enzyme form GluRSP
Triticum aestivum
6.1.1.17
164000
165000
non-denaturing PAGE, gel filtration, enzyme form GluRSE
Triticum aestivum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Triticum aestivum
-
enzyme forms: GuRSP and GluRSE
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.17
-
Triticum aestivum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.1.1.17
germ
2 enzyme forms: GluRSP and GluRSE
Triticum aestivum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
6.1.1.17
0.036
-
wheat germ enymes
Triticum aestivum
6.1.1.17
0.06
-
chloroplastic enzyme
Triticum aestivum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
223
Triticum aestivum
AMP + diphosphate + L-glutamyl-tRNAGlu
-
223
Triticum aestivum
-
Subunits
EC Number
Subunits
Commentary
Organism
6.1.1.17
dimer
2 * 55000, SDS-PAGE, chloroplastic enzyme; 2 * 79000, SDS-PAGE, enzyme form GluRSP; 2 * 83000, SDS-PAGE, enzyme form GluRSE
Triticum aestivum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.1.1.17
chloroplast
enzyme form GluRSC
Triticum aestivum
9507
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
55000
-
2 * 55000, SDS-PAGE, chloroplastic enzyme
Triticum aestivum
6.1.1.17
79000
-
2 * 79000, SDS-PAGE, enzyme form GluRSP
Triticum aestivum
6.1.1.17
83000
-
2 * 83000, SDS-PAGE, enzyme form GluRSE
Triticum aestivum
6.1.1.17
110000
112000
non-denaturing PAGE, gel filtration, chloroplastic enzyme
Triticum aestivum
6.1.1.17
155000
160000
gel filtration, non-denaturing PAGE, enzyme form GluRSP
Triticum aestivum
6.1.1.17
164000
165000
non-denaturing PAGE, gel filtration, enzyme form GluRSE
Triticum aestivum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.17
-
Triticum aestivum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.1.1.17
germ
2 enzyme forms: GluRSP and GluRSE
Triticum aestivum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
6.1.1.17
0.036
-
wheat germ enymes
Triticum aestivum
6.1.1.17
0.06
-
chloroplastic enzyme
Triticum aestivum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
223
Triticum aestivum
AMP + diphosphate + L-glutamyl-tRNAGlu
-
223
Triticum aestivum
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.1.1.17
dimer
2 * 55000, SDS-PAGE, chloroplastic enzyme; 2 * 79000, SDS-PAGE, enzyme form GluRSP; 2 * 83000, SDS-PAGE, enzyme form GluRSE
Triticum aestivum