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Literature summary extracted from

  • Ratinaud, M.H.; Thomes, J.C.; Julien, R.
    Glutamyl-tRNA synthetases from wheat. Isolation and characterization of three dimeric enzymes (1983), Eur. J. Biochem., 135, 471-477.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.17 chloroplast enzyme form GluRSC Triticum aestivum 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.17 55000
-
 2 * 55000, SDS-PAGE, chloroplastic enzyme Triticum aestivum
6.1.1.17 79000
-
 2 * 79000, SDS-PAGE, enzyme form GluRSP Triticum aestivum
6.1.1.17 83000
-
 2 * 83000, SDS-PAGE, enzyme form GluRSE Triticum aestivum
6.1.1.17 110000 112000 non-denaturing PAGE, gel filtration, chloroplastic enzyme Triticum aestivum
6.1.1.17 155000 160000 gel filtration, non-denaturing PAGE, enzyme form GluRSP Triticum aestivum
6.1.1.17 164000 165000 non-denaturing PAGE, gel filtration, enzyme form GluRSE Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.17 Triticum aestivum
-
 enzyme forms: GuRSP and GluRSE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.17
-
 Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.17 germ 2 enzyme forms: GluRSP and GluRSE Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.17 0.036
-
 wheat germ enymes Triticum aestivum
6.1.1.17 0.06
-
 chloroplastic enzyme Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Triticum aestivum AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.17 dimer 2 * 55000, SDS-PAGE, chloroplastic enzyme Triticum aestivum
6.1.1.17 dimer 2 * 83000, SDS-PAGE, enzyme form GluRSE Triticum aestivum
6.1.1.17 dimer 2 * 79000, SDS-PAGE, enzyme form GluRSP Triticum aestivum