BRENDA - Enzyme Database

The Monomeric glutamyl-tRNA synthetase of Escherichia coli. Purification and relation between its structural and catalytic properties

Kern, D.; Potier, S.; Boulanger, Y.; Lapointe, J.; J. Biol. Chem. 254, 518-524 (1979)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.1.1.17
p-hydroxymercuribenzoate
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.00008
-
tRNAGlu
-
Escherichia coli
6.1.1.17
0.1
-
ATP
Glu
Escherichia coli
6.1.1.17
0.18
-
ATP
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
56000
-
1 * 56000, SDS-PAGE
Escherichia coli
6.1.1.17
58000
-
electrophoresis in polyacrylamide gels of various concentrations
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.17
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.1.1.17
1.071
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
216
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
216
Escherichia coli
-
6.1.1.17
additional information
tRNAGlu-dependent ATP-diphosphate exchange
216
Escherichia coli
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
6.1.1.17
monomer
1 * 56000, SDS-PAGE
Escherichia coli
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.1.1.17
3.4
-
Glu-tRNA
-
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1.1.17
6.2
-
tRNAGlu-dependent ATP-diphosphate exchange
Escherichia coli
6.1.1.17
8.6
-
glutamyl-tRNA formation
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.1.1.17
p-hydroxymercuribenzoate
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.00008
-
tRNAGlu
-
Escherichia coli
6.1.1.17
0.1
-
ATP
Glu
Escherichia coli
6.1.1.17
0.18
-
ATP
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.1.1.17
56000
-
1 * 56000, SDS-PAGE
Escherichia coli
6.1.1.17
58000
-
electrophoresis in polyacrylamide gels of various concentrations
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.17
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.1.1.17
1.071
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
216
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
216
Escherichia coli
-
6.1.1.17
additional information
tRNAGlu-dependent ATP-diphosphate exchange
216
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.1.1.17
monomer
1 * 56000, SDS-PAGE
Escherichia coli
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.1.1.17
3.4
-
Glu-tRNA
-
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1.1.17
6.2
-
tRNAGlu-dependent ATP-diphosphate exchange
Escherichia coli
6.1.1.17
8.6
-
glutamyl-tRNA formation
Escherichia coli