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Literature summary extracted from

  • Guillouet, S.; Rodal, A.A.; An, G.H.; Lessard, P.A.; Sinskey, A.J.
    Expression of the Escherichia coli catabolic threonine dehydratase in Corynebacterium glutamicum and its effect on isoleucine production (1999), Appl. Environ. Microbiol., 65, 3100-3107.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.19 expression in Brevibacterium flavum Escherichia coli
4.3.1.19 expression in Corynebacterium glutamicum Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.19 Ile native enzyme is totally inhibited by 15 mM Ile, the heterologous catabolic enzyme from E. coli retains 60% of its original activity even in presence of 200 mM Ile Corynebacterium glutamicum
4.3.1.19 Ile biodegradative enzyme is feedback-resistant Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.19 L-Thr Escherichia coli first reaction of the Ile pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.19 Corynebacterium glutamicum
-
-
-
4.3.1.19 Escherichia coli
-
 biodegradative threonine dehydratase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.19 L-Thr first reaction of the Ile pathway Escherichia coli ?
-
 ?
4.3.1.19 L-threonine
-
 Escherichia coli 2-oxobutanoate + NH3
-
 ?
4.3.1.19 L-threonine
-
 Corynebacterium glutamicum 2-oxobutanoate + NH3
-
 ?