Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Robinson, W.G.; Labow, R.
    D-Serine dehydratase (Escherichia coli) (1971), Methods Enzymol., 17B, 356-360.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.1.18
-
 Escherichia coli

General Stability

EC Number General Stability Organism
4.3.1.18 repeated freezing and thawing over a period of 1 or 2 months causes a 20-30% loss of activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.18 0.7
-
 D-Ser
-
 Escherichia coli
4.3.1.18 0.92
-
 D-Thr
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.18 37300
-
 analytical ultracentrifugation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.18 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.18
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
4.3.1.18 0°C, stable almost indefinitely Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.18 D-serine
-
 Escherichia coli pyruvate + NH3
-
 ?
4.3.1.18 D-threonine
-
 Escherichia coli 2-oxobutanoate + NH3
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.18 22
-
 6 h, 10% loss of activity Escherichia coli
4.3.1.18 54
-
 1 min, dilute solution, complete loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.18 8
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.18 pyridoxal 5'-phosphate 1 mol of pyridoxal 5'-phosphate is bound per mol of enzyme to one epsilon-amino group Escherichia coli