Literature summary extracted from

Kervinen, J.; Dunbrack, R.L.; Litwin, S.; Martins, J.; Scarrow, R.C.; Volin, M.; Yeung, A.T.; Yoon, E.; Jaffe, E.K.
Porphobilinogen synthase from pea: expression from an artificial gene, kinetic characterization, and novel implications for subunit interactions (2000), Biochemistry, 39, 9018-9029.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.2.1.24	expression in Escherichia coli	Pisum sativum

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
4.2.1.24	succinylacetone	-	Pisum sativum

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
4.2.1.24	Mg2+	essentail cofactor, allosteric Mg(II) binds with a Kd of 2.5 mM, 2.3fold activation, binding of 3 Mg(II) per subunit	Pisum sativum

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
4.2.1.24	37000	-	x * 37000, maximally active octamer can dissociate into less active smaller subunits, minimal functional unit is a tetramer, SDS-PAGE	Pisum sativum

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.1.24	Pisum sativum	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.2.1.24	-	Pisum sativum

Subunits

EC Number	Subunits	Comment	Organism
4.2.1.24	?	x * 37000, maximally active octamer can dissociate into less active smaller subunits, minimal functional unit is a tetramer, SDS-PAGE	Pisum sativum

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.2.1.24	7.7	9.2	-	Pisum sativum

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Release 2023.1 (January 2023)