BRENDA - Enzyme Database show

Purification and characterization of 5-aminolevulinic acid dehydratase from Methanosarcina barkeri

Bhosale, S.; Kshirsagar, D.; Pawar, P.; Yeole, T.; Ranade, D.; FEMS Microbiol. Lett. 127, 151-155 (1995)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
Cu2+
-
Methanosarcina barkeri
4.2.1.24
Pb2+
-
Methanosarcina barkeri
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
K+
activates up to 3 mM
Methanosarcina barkeri
4.2.1.24
Ni(2+)
0.15 mM, activates
Methanosarcina barkeri
4.2.1.24
Zn2+
activates; optimal stimulation at 0.1 mM
Methanosarcina barkeri
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
30000
-
8 * 30000, SDS-PAGE
Methanosarcina barkeri
4.2.1.24
280000
-
gel filtration
Methanosarcina barkeri
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Methanosarcina barkeri
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.24
-
Methanosarcina barkeri
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.24
0.39
-
-
Methanosarcina barkeri
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210715
Methanosarcina barkeri
porphobilinogen + 2 H2O
-
210715
Methanosarcina barkeri
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.24
octamer
8 * 30000, SDS-PAGE
Methanosarcina barkeri
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
8.5
-
two pH-optima at pH 8.5 and at pH 9.4
Methanosarcina barkeri
4.2.1.24
9.4
-
two pH-optima at pH 8.5 and at pH 9.4
Methanosarcina barkeri
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
Cu2+
-
Methanosarcina barkeri
4.2.1.24
Pb2+
-
Methanosarcina barkeri
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
K+
activates up to 3 mM
Methanosarcina barkeri
4.2.1.24
Ni(2+)
0.15 mM, activates
Methanosarcina barkeri
4.2.1.24
Zn2+
activates; optimal stimulation at 0.1 mM
Methanosarcina barkeri
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
30000
-
8 * 30000, SDS-PAGE
Methanosarcina barkeri
4.2.1.24
280000
-
gel filtration
Methanosarcina barkeri
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
-
Methanosarcina barkeri
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.24
0.39
-
-
Methanosarcina barkeri
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210715
Methanosarcina barkeri
porphobilinogen + 2 H2O
-
210715
Methanosarcina barkeri
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.24
octamer
8 * 30000, SDS-PAGE
Methanosarcina barkeri
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
8.5
-
two pH-optima at pH 8.5 and at pH 9.4
Methanosarcina barkeri
4.2.1.24
9.4
-
two pH-optima at pH 8.5 and at pH 9.4
Methanosarcina barkeri