BRENDA - Enzyme Database show

Beef-liver 5-aminolevulinic acid dehydratase

Wilson, E.L.; Burger, P.E.; Dowdle, E.B.; Eur. J. Biochem. 29, 563-671 (1972)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.24
thiol
required
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
Cd2+
inhibition at low concentration of substrate and stimulation at high levels of substrate
Bos taurus
4.2.1.24
EDTA
-
Bos taurus
4.2.1.24
iodoacetamide
1 mM, 97% inhibition
Bos taurus
4.2.1.24
NEM
1 mM, 85% inhibition
Bos taurus
4.2.1.24
Pb2+
-
Bos taurus
4.2.1.24
PCMB
1 mM, 97% inhibition
Bos taurus
4.2.1.24
Zn2+
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.15
-
5-aminolevulinate
partially purified enzyme
Bos taurus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Cd2+
inhibition at low concentration of substrate and stimulation at high levels of substrate
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
260000
-
low-speed equilibrium sedimentation
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.24
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.24
liver
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210709
Bos taurus
porphobilinogen + 2 H2O
-
210709
Bos taurus
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.24
More
-
Bos taurus
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.24
65
-
-
Bos taurus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.2.1.24
64
-
60 min, 20% loss of activity
Bos taurus
4.2.1.24
80
-
15 min, 50% loss of activity
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
6.3
6.4
-
Bos taurus
4.2.1.24
6.3
-
-
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.24
thiol
required
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
Cd2+
inhibition at low concentration of substrate and stimulation at high levels of substrate
Bos taurus
4.2.1.24
EDTA
-
Bos taurus
4.2.1.24
iodoacetamide
1 mM, 97% inhibition
Bos taurus
4.2.1.24
NEM
1 mM, 85% inhibition
Bos taurus
4.2.1.24
Pb2+
-
Bos taurus
4.2.1.24
PCMB
1 mM, 97% inhibition
Bos taurus
4.2.1.24
Zn2+
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.15
-
5-aminolevulinate
partially purified enzyme
Bos taurus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Cd2+
inhibition at low concentration of substrate and stimulation at high levels of substrate
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
260000
-
low-speed equilibrium sedimentation
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.24
liver
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210709
Bos taurus
porphobilinogen + 2 H2O
-
210709
Bos taurus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.24
More
-
Bos taurus
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.24
65
-
-
Bos taurus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.2.1.24
64
-
60 min, 20% loss of activity
Bos taurus
4.2.1.24
80
-
15 min, 50% loss of activity
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
6.3
6.4
-
Bos taurus
4.2.1.24
6.3
-
-
Bos taurus