BRENDA - Enzyme Database show

Purification, properties and synthesis of delta-aminolaevulinate dehydratase from Neurospora crassa

Chandrika, S.R.; Padmanaban, G.; Biochem. J. 191, 29-36 (1980)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
Coproporphyrinogen III
-
Neurospora crassa
4.2.1.24
p-hydroxymercuribenzoate
-
Neurospora crassa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.1
-
5-aminolevulinate
-
Neurospora crassa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
38000
-
x * 38000, SDS-PAGE
Neurospora crassa
4.2.1.24
350000
-
gel filtration
Neurospora crassa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.24
5-aminolevulinate
Neurospora crassa
the second and rate-limiting enzyme of the heme-biosynthetic pathway
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Neurospora crassa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.24
-
Neurospora crassa
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.24
additional information
-
-
Neurospora crassa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate
the second and rate-limiting enzyme of the heme-biosynthetic pathway
210697
Neurospora crassa
?
-
-
-
-
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210697
Neurospora crassa
porphobilinogen + 2 H2O
-
210697
Neurospora crassa
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.24
?
x * 38000, SDS-PAGE
Neurospora crassa
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
4.2.1.24
55
-
-
Neurospora crassa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
8
8.4
-
Neurospora crassa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
Coproporphyrinogen III
-
Neurospora crassa
4.2.1.24
p-hydroxymercuribenzoate
-
Neurospora crassa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.1
-
5-aminolevulinate
-
Neurospora crassa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
38000
-
x * 38000, SDS-PAGE
Neurospora crassa
4.2.1.24
350000
-
gel filtration
Neurospora crassa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.24
5-aminolevulinate
Neurospora crassa
the second and rate-limiting enzyme of the heme-biosynthetic pathway
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
-
Neurospora crassa
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.24
additional information
-
-
Neurospora crassa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate
the second and rate-limiting enzyme of the heme-biosynthetic pathway
210697
Neurospora crassa
?
-
-
-
-
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210697
Neurospora crassa
porphobilinogen + 2 H2O
-
210697
Neurospora crassa
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.24
?
x * 38000, SDS-PAGE
Neurospora crassa
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
4.2.1.24
55
-
-
Neurospora crassa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.24
8
8.4
-
Neurospora crassa