BRENDA - Enzyme Database show

Purification of porphobilinogen synthase from bovine liver

Jordan, P.M.; Seehra, J.S.; Methods Enzymol. 123, 427-434 (1986)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.24
thiol
20 mM, essential to maintain the enzyme in its reduced state
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
5-bromolevulinic acid
-
Bos taurus
4.2.1.24
5-chlorolevulinic acid
-
Bos taurus
4.2.1.24
5-iodolevulinic acid
-
Bos taurus
4.2.1.24
DTNB
reversible loss of activity
Bos taurus
4.2.1.24
EDTA
-
Bos taurus
4.2.1.24
iodoacetamide
irreversible
Bos taurus
4.2.1.24
iodoacetate
irreversible
Bos taurus
4.2.1.24
Mercury ions
-
Bos taurus
4.2.1.24
Pb2+
-
Bos taurus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Zn2+
required
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
35000
-
8 * 35000, SDS-PAGE
Bos taurus
4.2.1.24
280000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.24
5-aminolevulinate
Bos taurus
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Bos taurus
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
4.2.1.24
total but reversible loss of activity by O2
Bos taurus
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.24
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.24
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.24
0.63
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate
-
210686
Bos taurus
?
-
-
-
-
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210686
Bos taurus
porphobilinogen + 2 H2O
-
210686
Bos taurus
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.24
octamer
8 * 35000, SDS-PAGE
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.24
thiol
20 mM, essential to maintain the enzyme in its reduced state
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
5-bromolevulinic acid
-
Bos taurus
4.2.1.24
5-chlorolevulinic acid
-
Bos taurus
4.2.1.24
5-iodolevulinic acid
-
Bos taurus
4.2.1.24
DTNB
reversible loss of activity
Bos taurus
4.2.1.24
EDTA
-
Bos taurus
4.2.1.24
iodoacetamide
irreversible
Bos taurus
4.2.1.24
iodoacetate
irreversible
Bos taurus
4.2.1.24
Mercury ions
-
Bos taurus
4.2.1.24
Pb2+
-
Bos taurus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.24
Zn2+
required
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.24
35000
-
8 * 35000, SDS-PAGE
Bos taurus
4.2.1.24
280000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.24
5-aminolevulinate
Bos taurus
-
?
-
-
-
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
4.2.1.24
total but reversible loss of activity by O2
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.24
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.24
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.2.1.24
0.63
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate
-
210686
Bos taurus
?
-
-
-
-
4.2.1.24
5-aminolevulinate + 5-aminolevulinate
-
210686
Bos taurus
porphobilinogen + 2 H2O
-
210686
Bos taurus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.24
octamer
8 * 35000, SDS-PAGE
Bos taurus