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Literature summary extracted from

  • Battastini, A.M.O.; Oliveira, E.M.; Moreira, C.M.; Bonan, C.D; Sarkis, J.J; Dias, R.D.
    Solubilization and characterization of an ATP diphosphohydrolase (EC 3.6.1.5) from rat brain synaptic plasma membranes (1995), Biochem. Mol. Biol. Int., 37, 209-219.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.6.1.5 the detergents Triton X-100, deoxycholate, CHAPS, Nonidet, N-octoglucoside, C12E8 inactivate the enzyme, digitonin is not harmful Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.5 additional information no inhibition by oligomycin; no inhibition by orthovanadate; no inhibition by ouabain Rattus norvegicus
3.6.1.5 NaN3
-
 Rattus norvegicus
3.6.1.5 NEM
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.5 membrane synaptic plasma membrane Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.5 Ca2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.5 60000 70000 immunoblotting Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.5 ADP + H2O Rattus norvegicus
-
 AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O Rattus norvegicus
-
 AMP + 2 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.5 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.5 partially Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.5 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.5 0.854
-
 partially purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.5 ADP + H2O
-
 Rattus norvegicus AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O
-
 Rattus norvegicus AMP + 2 phosphate
-
 ?
3.6.1.5 additional information neglible hydrolysis of AMP Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.5 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.5 8
-
-
 Rattus norvegicus