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Literature summary extracted from

  • Barchi, R.L.
    Thiamine triphosphatases in brain (1974), Thiamine (Proc. Pap. U. S. -Jpn. Semin, 2. Meeting), , 195-212.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.28 ADP 1 mM, strongly inhibits membrane-associated enzyme, soluble enzyme is unaffected Rattus norvegicus
3.6.1.28 ATP 1 mM, strongly inhibits membrane-associated enzyme, soluble enzyme is unaffected Rattus norvegicus
3.6.1.28 Ca2+ 0.1-1.0 mM, in presence of Mg2+, soluble enzymem Rattus norvegicus
3.6.1.28 thiamine diphosphate soluble enzyme, at levels of substrate 2 to 3 times Km, no inhibition of membrane-associated enzyme Rattus norvegicus
3.6.1.28 thiamine triphosphate substrate inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.28 0.6
-
 Mg2+-thiamine triphosphate complex
-
 Rattus norvegicus
3.6.1.28 1.2
-
 thiamine triphosphate
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.28 Ca2+ divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+ Rattus norvegicus
3.6.1.28 Ca2+ activates membrane-associated enzyme Rattus norvegicus
3.6.1.28 Mg2+ maximal activation occurs at a cation to substrate ratio of 1:1 Rattus norvegicus
3.6.1.28 Mg2+ divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+ Rattus norvegicus
3.6.1.28 Mn2+ divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+ Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.28 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.28 brain
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.28 thiamine triphosphate + H2O
-
 Rattus norvegicus thiamine diphosphate + phosphate
-
 ?
3.6.1.28 thiamine triphosphate + H2O thiamine triphosphate as Mg2+-thiamine triphosphate complex Rattus norvegicus thiamine diphosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.28 50
-
 soluble enzyme Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.28 50
-
 rapid inactivation of membrane-associated enzyme Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.28 6.5 7 membrane-associated enzyme Rattus norvegicus
3.6.1.28 9 9.5 soluble enzyme Rattus norvegicus