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Literature summary extracted from
- A membrane-associated thiamine triphosphatase from rat brain. Properties of the enzyme (1972), J. Biol. Chem., 247, 7668-7673.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.28 | ADP | 0.001-1 mM, strong inhibition | Rattus norvegicus |  |
| 3.6.1.28 | deoxycholate | - |
Rattus norvegicus | |
| 3.6.1.28 | thiamine beta,gamma-methylene phosphonate | - |
Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.28 | 1.4 | - |
thiamine triphosphate | soluble enzyme, activated by Mg2+ | Rattus norvegicus | |
| 3.6.1.28 | 1.5 | - |
thiamine triphosphate | membrane-associated enzyme, activated by Ca2+ or Mg2+ | Rattus norvegicus | |
| 3.6.1.28 | 2 | - |
thiamine triphosphate | soluble enzyme, activated by Ca2+ | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.6.1.28 | membrane | associated | Rattus norvegicus | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.28 | Br- | stimulation by anions with the following order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme | Rattus norvegicus | |
| 3.6.1.28 | Ca2+ | maximal activation occurs at a cation to substrate ratio of 1:1 | Rattus norvegicus | |
| 3.6.1.28 | Ca2+ | divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+ | Rattus norvegicus | |
| 3.6.1.28 | Cl- | stimulation by anions with the following decreasing order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme | Rattus norvegicus | |
| 3.6.1.28 | I- | stimulation by anions with the following decreasing order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme | Rattus norvegicus | |
| 3.6.1.28 | Mg2+ | maximal activation occurs at a cation to substrate ratio of 1:1 | Rattus norvegicus | |
| 3.6.1.28 | Mg2+ | divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+ | Rattus norvegicus | |
| 3.6.1.28 | Mn2+ | maximal activation occurs at a cation to substrate ratio of 1:1 | Rattus norvegicus | |
| 3.6.1.28 | Mn2+ | divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+ | Rattus norvegicus | |
| 3.6.1.28 | NO3- | stimulation by anions with the following decreasing order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme. The effect of NO3- is maximal at pH 7-8, it is negligible at pH 6.5 | Rattus norvegicus | |
| 3.6.1.28 | SCN- | stimulation by anions with the following decreasing order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.28 | 30000 | - |
enzyme from liver and brain, gel filtration | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.28 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.28 | partial | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.6.1.28 | brain | - |
Rattus norvegicus | - |
| 3.6.1.28 | heart | - |
Rattus norvegicus | - |
| 3.6.1.28 | intestine | - |
Rattus norvegicus | - |
| 3.6.1.28 | kidney | - |
Rattus norvegicus | - |
| 3.6.1.28 | liver | - |
Rattus norvegicus | - |
| 3.6.1.28 | skeletal muscle | - |
Rattus norvegicus | - |
| 3.6.1.28 | spleen | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.28 | thiamine triphosphate + H2O | - |
Rattus norvegicus | thiamine diphosphate + phosphate | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.28 | 50 | - |
50% inactivation after 2-4 min | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.28 | 6.5 | - |
membrane-associated enzyme | Rattus norvegicus |
| 3.6.1.28 | 9.5 | - |
soluble enzyme | Rattus norvegicus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.28 | 5 | 8 | about 55% of maximal activity at pH 5.0 and pH 8.0, membrane-associated enzyme | Rattus norvegicus |
| 3.6.1.28 | 8 | 10 | pH 8.0: about 60% of maximal activity, pH 10.0: about 75% of maximal activity | Rattus norvegicus |
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Release 2023.1 (January 2023)
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