BRENDA - Enzyme Database

Cloning and characterization of the NADH pyrophospatases from Caenorhabditis elegans and Saccharomyces cerevisiae, members of a nudix hydrolase subfamily

Xu, W.; Dunn, C.A.; Bessman, M.J.; Biochem. Biophys. Res. Commun. 273, 753-758 (2000)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.6.1.22
expressed in Escherichia coli
Caenorhabditis elegans
3.6.1.22
expressed in Escherichia coli
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.22
Ca2+
in presence of Mg2+ complete inhibition
Caenorhabditis elegans
3.6.1.22
Ca2+
in presence of Mg2+ complete inhibition
Saccharomyces cerevisiae
3.6.1.22
Zn2+
in presence of Mg2+ complete inhibition
Caenorhabditis elegans
3.6.1.22
Zn2+
in presence of Mg2+ complete inhibition
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.22
1.4
-
NADH
50 mM Tris-HCl, pH 8.5, 5 mM MgCl2
Caenorhabditis elegans
3.6.1.22
1.6
-
NADH
50 mM Tris-HCl, pH 7.5, 5 mM MgCl2
Saccharomyces cerevisiae
3.6.1.22
6.6
-
NAD+
50 mM Tris-HCl, pH 8.5, 5 mM MgCl2
Caenorhabditis elegans
3.6.1.22
6.6
-
NAD+
50 mM Tris-HCl, pH 7.5, 5 mM MgCl2
Saccharomyces cerevisiae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.22
Mg2+
strong activation
Caenorhabditis elegans
3.6.1.22
Mg2+
strong activation
Escherichia coli
3.6.1.22
Mg2+
strong activation
Saccharomyces cerevisiae
3.6.1.22
Mn2+
strong activation
Caenorhabditis elegans
3.6.1.22
Mn2+
strong activation
Escherichia coli
3.6.1.22
Mn2+
strong activation
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.22
39100
-
homodimer, 2 * 39100
Caenorhabditis elegans
3.6.1.22
43500
-
homodimer, 2 * 43500
Saccharomyces cerevisiae
3.6.1.22
78200
-
two subunits of 39100 Da found
Caenorhabditis elegans
3.6.1.22
87000
-
two subunits of 43500 Da found
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.22
NADH + H2O
Escherichia coli
possible function in regulation of NAD+/NADH-ratio
NMNH + AMP
-
Escherichia coli
ir
3.6.1.22
NADH + H2O
Saccharomyces cerevisiae
possible function in regulation of NAD+/NADH-ratio
NMNH + AMP
-
Saccharomyces cerevisiae
ir
3.6.1.22
NADH + H2O
Caenorhabditis elegans
possible function in regulation of NAD+/NADH-ratio
NMNH + AMP
-
Caenorhabditis elegans
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.22
Caenorhabditis elegans
-
-
-
3.6.1.22
Escherichia coli
-
-
-
3.6.1.22
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.6.1.22
purification of gene product from Escherichia coli
Caenorhabditis elegans
3.6.1.22
purification of gene product from Escherichia coli
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.22
ADPribose + H2O
-
209959
Escherichia coli
?
-
-
-
?
3.6.1.22
ADPribose + H2O
-
209959
Saccharomyces cerevisiae
?
-
-
-
?
3.6.1.22
ADPribose + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
3.6.1.22
AppA + H2O
-
209959
Escherichia coli
?
-
-
-
?
3.6.1.22
AppA + H2O
-
209959
Saccharomyces cerevisiae
?
-
-
-
?
3.6.1.22
AppA + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
3.6.1.22
dATP + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
3.6.1.22
NAD+ + H2O
-
209959
Escherichia coli
NMN + AMP
-
-
-
ir
3.6.1.22
NAD+ + H2O
-
209959
Saccharomyces cerevisiae
NMN + AMP
-
-
-
ir
3.6.1.22
NAD+ + H2O
-
209959
Caenorhabditis elegans
NMN + AMP
-
-
-
ir
3.6.1.22
NADH + H2O
possible function in regulation of NAD+/NADH-ratio
209959
Escherichia coli
NMNH + AMP
-
209959
Escherichia coli
ir
3.6.1.22
NADH + H2O
possible function in regulation of NAD+/NADH-ratio
209959
Saccharomyces cerevisiae
NMNH + AMP
-
209959
Saccharomyces cerevisiae
ir
3.6.1.22
NADH + H2O
possible function in regulation of NAD+/NADH-ratio
209959
Caenorhabditis elegans
NMNH + AMP
-
209959
Caenorhabditis elegans
ir
3.6.1.22
NADH + H2O
-
209959
Escherichia coli
AMP + NMNH
-
209959
Escherichia coli
?
3.6.1.22
NADH + H2O
-
209959
Saccharomyces cerevisiae
AMP + NMNH
-
209959
Saccharomyces cerevisiae
?
3.6.1.22
NADH + H2O
-
209959
Caenorhabditis elegans
AMP + NMNH
-
209959
Caenorhabditis elegans
?
3.6.1.22
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209959
Escherichia coli
?
-
-
-
?
3.6.1.22
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209959
Saccharomyces cerevisiae
?
-
-
-
?
3.6.1.22
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.6.1.22
dimer
homodimer, 2 * 39100
Caenorhabditis elegans
3.6.1.22
dimer
-
Escherichia coli
3.6.1.22
dimer
homodimer, 2 * 43500
Saccharomyces cerevisiae
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.22
0.000167
-
NAD+
-
Caenorhabditis elegans
3.6.1.22
0.000167
-
NAD+
-
Saccharomyces cerevisiae
3.6.1.22
0.00117
-
NADH
-
Caenorhabditis elegans
3.6.1.22
0.0025
-
NADH
-
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.22
7.5
-
-
Saccharomyces cerevisiae
3.6.1.22
8.5
-
-
Caenorhabditis elegans
3.6.1.22
8.5
-
-
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.22
expressed in Escherichia coli
Caenorhabditis elegans
3.6.1.22
expressed in Escherichia coli
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.22
Ca2+
in presence of Mg2+ complete inhibition
Caenorhabditis elegans
3.6.1.22
Ca2+
in presence of Mg2+ complete inhibition
Saccharomyces cerevisiae
3.6.1.22
Zn2+
in presence of Mg2+ complete inhibition
Caenorhabditis elegans
3.6.1.22
Zn2+
in presence of Mg2+ complete inhibition
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.22
1.4
-
NADH
50 mM Tris-HCl, pH 8.5, 5 mM MgCl2
Caenorhabditis elegans
3.6.1.22
1.6
-
NADH
50 mM Tris-HCl, pH 7.5, 5 mM MgCl2
Saccharomyces cerevisiae
3.6.1.22
6.6
-
NAD+
50 mM Tris-HCl, pH 8.5, 5 mM MgCl2
Caenorhabditis elegans
3.6.1.22
6.6
-
NAD+
50 mM Tris-HCl, pH 7.5, 5 mM MgCl2
Saccharomyces cerevisiae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.22
Mg2+
strong activation
Caenorhabditis elegans
3.6.1.22
Mg2+
strong activation
Escherichia coli
3.6.1.22
Mg2+
strong activation
Saccharomyces cerevisiae
3.6.1.22
Mn2+
strong activation
Caenorhabditis elegans
3.6.1.22
Mn2+
strong activation
Escherichia coli
3.6.1.22
Mn2+
strong activation
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.22
39100
-
homodimer, 2 * 39100
Caenorhabditis elegans
3.6.1.22
43500
-
homodimer, 2 * 43500
Saccharomyces cerevisiae
3.6.1.22
78200
-
two subunits of 39100 Da found
Caenorhabditis elegans
3.6.1.22
87000
-
two subunits of 43500 Da found
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.22
NADH + H2O
Escherichia coli
possible function in regulation of NAD+/NADH-ratio
NMNH + AMP
-
Escherichia coli
ir
3.6.1.22
NADH + H2O
Saccharomyces cerevisiae
possible function in regulation of NAD+/NADH-ratio
NMNH + AMP
-
Saccharomyces cerevisiae
ir
3.6.1.22
NADH + H2O
Caenorhabditis elegans
possible function in regulation of NAD+/NADH-ratio
NMNH + AMP
-
Caenorhabditis elegans
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.22
purification of gene product from Escherichia coli
Caenorhabditis elegans
3.6.1.22
purification of gene product from Escherichia coli
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.22
ADPribose + H2O
-
209959
Escherichia coli
?
-
-
-
?
3.6.1.22
ADPribose + H2O
-
209959
Saccharomyces cerevisiae
?
-
-
-
?
3.6.1.22
ADPribose + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
3.6.1.22
AppA + H2O
-
209959
Escherichia coli
?
-
-
-
?
3.6.1.22
AppA + H2O
-
209959
Saccharomyces cerevisiae
?
-
-
-
?
3.6.1.22
AppA + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
3.6.1.22
dATP + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
3.6.1.22
NAD+ + H2O
-
209959
Escherichia coli
NMN + AMP
-
-
-
ir
3.6.1.22
NAD+ + H2O
-
209959
Saccharomyces cerevisiae
NMN + AMP
-
-
-
ir
3.6.1.22
NAD+ + H2O
-
209959
Caenorhabditis elegans
NMN + AMP
-
-
-
ir
3.6.1.22
NADH + H2O
possible function in regulation of NAD+/NADH-ratio
209959
Escherichia coli
NMNH + AMP
-
209959
Escherichia coli
ir
3.6.1.22
NADH + H2O
possible function in regulation of NAD+/NADH-ratio
209959
Saccharomyces cerevisiae
NMNH + AMP
-
209959
Saccharomyces cerevisiae
ir
3.6.1.22
NADH + H2O
possible function in regulation of NAD+/NADH-ratio
209959
Caenorhabditis elegans
NMNH + AMP
-
209959
Caenorhabditis elegans
ir
3.6.1.22
NADH + H2O
-
209959
Escherichia coli
AMP + NMNH
-
209959
Escherichia coli
?
3.6.1.22
NADH + H2O
-
209959
Saccharomyces cerevisiae
AMP + NMNH
-
209959
Saccharomyces cerevisiae
?
3.6.1.22
NADH + H2O
-
209959
Caenorhabditis elegans
AMP + NMNH
-
209959
Caenorhabditis elegans
?
3.6.1.22
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209959
Escherichia coli
?
-
-
-
?
3.6.1.22
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209959
Saccharomyces cerevisiae
?
-
-
-
?
3.6.1.22
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.6.1.22
dimer
homodimer, 2 * 39100
Caenorhabditis elegans
3.6.1.22
dimer
-
Escherichia coli
3.6.1.22
dimer
homodimer, 2 * 43500
Saccharomyces cerevisiae
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.22
0.000167
-
NAD+
-
Caenorhabditis elegans
3.6.1.22
0.000167
-
NAD+
-
Saccharomyces cerevisiae
3.6.1.22
0.00117
-
NADH
-
Caenorhabditis elegans
3.6.1.22
0.0025
-
NADH
-
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.22
7.5
-
-
Saccharomyces cerevisiae
3.6.1.22
8.5
-
-
Caenorhabditis elegans
3.6.1.22
8.5
-
-
Escherichia coli