BRENDA - Enzyme Database

Human placental (asymmetrical) diadenosine 5',5'''-P1,P4-tetraphosphate hydrolase: Purification to homogenity and some properties

Lazewska, D.; Starzynska, E.; Guranowski, A.; Protein Expr. Purif. 4, 45-51 (1993)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.17
adenosine 5'-tetraphosphate
Ki: 0.006 mM
Homo sapiens
3.6.1.17
Ca2+
Ki: 0.3 mM
Homo sapiens
3.6.1.17
diadenosine triphosphate
competitive, also phosphonate analogues of the substance
Homo sapiens
3.6.1.17
fluoride
Ki: 0.02 mM
Homo sapiens
3.6.1.17
guanosine 5'-tetraphosphate
Ki: 0.01 mM
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.01
-
P1,P4-bis(5'-adenosyl) tetraphosphate
in presence of Mg2+
Homo sapiens
3.6.1.17
0.04
-
P1,P4-bis(5'-adenosyl) tetraphosphate
in presence of Zn2+
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.17
Co2+
60% of activity
Homo sapiens
3.6.1.17
Mg2+
maximum activity at 3 mM
Homo sapiens
3.6.1.17
Mn2+
40% of activity
Homo sapiens
3.6.1.17
Zn2+
50% of activity
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.17
19200
-
SDS-PAGE, gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Homo sapiens
-
ATP + AMP
-
Homo sapiens
?
3.6.1.17
P1,P4-bis(5'-guanosyl)tetraphosphate + H2O
Homo sapiens
-
GTP + GMP
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.17
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.6.1.17
-
Homo sapiens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.17
0.00022
-
crude extract
Homo sapiens
3.6.1.17
52
-
purified enzyme
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
di-2,6-diaminopurine beta-D-ribofuranoside tetraphosphate + H2O
-
209908
Homo sapiens
?
-
-
-
?
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209908
Homo sapiens
ATP + AMP
-
209908
Homo sapiens
?
3.6.1.17
P1,P4-bis(5'-guanosyl)tetraphosphate + H2O
-
209908
Homo sapiens
GTP + GMP
-
209908
Homo sapiens
?
3.6.1.17
P1,P5-bis(5'-adenosyl) pentaphosphate + H2O
-
209908
Homo sapiens
ADP + ATP
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.17
7.3
-
-
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.6.1.17
6.5
8.5
half maximum activity
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.6.1.17
0.01
-
guanosine 5'-tetraphosphate
-
Homo sapiens
3.6.1.17
0.02
-
fluoride
-
Homo sapiens
3.6.1.17
0.3
-
Ca2+
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.6.1.17
adenosine 5'-tetraphosphate
Ki: 0.006 mM
Homo sapiens
3.6.1.17
Ca2+
Ki: 0.3 mM
Homo sapiens
3.6.1.17
diadenosine triphosphate
competitive, also phosphonate analogues of the substance
Homo sapiens
3.6.1.17
fluoride
Ki: 0.02 mM
Homo sapiens
3.6.1.17
guanosine 5'-tetraphosphate
Ki: 0.01 mM
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.6.1.17
0.01
-
guanosine 5'-tetraphosphate
-
Homo sapiens
3.6.1.17
0.02
-
fluoride
-
Homo sapiens
3.6.1.17
0.3
-
Ca2+
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.01
-
P1,P4-bis(5'-adenosyl) tetraphosphate
in presence of Mg2+
Homo sapiens
3.6.1.17
0.04
-
P1,P4-bis(5'-adenosyl) tetraphosphate
in presence of Zn2+
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.17
Co2+
60% of activity
Homo sapiens
3.6.1.17
Mg2+
maximum activity at 3 mM
Homo sapiens
3.6.1.17
Mn2+
40% of activity
Homo sapiens
3.6.1.17
Zn2+
50% of activity
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.17
19200
-
SDS-PAGE, gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
Homo sapiens
-
ATP + AMP
-
Homo sapiens
?
3.6.1.17
P1,P4-bis(5'-guanosyl)tetraphosphate + H2O
Homo sapiens
-
GTP + GMP
-
Homo sapiens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.17
-
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.6.1.17
0.00022
-
crude extract
Homo sapiens
3.6.1.17
52
-
purified enzyme
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
di-2,6-diaminopurine beta-D-ribofuranoside tetraphosphate + H2O
-
209908
Homo sapiens
?
-
-
-
?
3.6.1.17
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209908
Homo sapiens
ATP + AMP
-
209908
Homo sapiens
?
3.6.1.17
P1,P4-bis(5'-guanosyl)tetraphosphate + H2O
-
209908
Homo sapiens
GTP + GMP
-
209908
Homo sapiens
?
3.6.1.17
P1,P5-bis(5'-adenosyl) pentaphosphate + H2O
-
209908
Homo sapiens
ADP + ATP
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.17
7.3
-
-
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.6.1.17
6.5
8.5
half maximum activity
Homo sapiens