BRENDA - Enzyme Database show

Cytidine deaminases (from Escherichia coli and human liver)

Wentworth, D.F.; Wolfenden, R.; Methods Enzymol. 51, 401-407 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
5,6-Dihydrouridine
competitive inhibition
Escherichia coli
3.5.4.5
5,6-Dihydrouridine
-
Homo sapiens
3.5.4.5
uridine
-
Escherichia coli
3.5.4.5
uridine
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.0092
-
cytidine
-
Homo sapiens
3.5.4.5
0.058
-
5-azacytidine
-
Homo sapiens
3.5.4.5
0.089
-
cytosine deoxyriboside
-
Escherichia coli
3.5.4.5
0.11
-
5,6-Dihydrocytidine
-
Escherichia coli
3.5.4.5
0.14
-
cytosine arabinoside
-
Homo sapiens
3.5.4.5
0.21
-
cytidine
-
Escherichia coli
3.5.4.5
4.2
-
6-azacytidine
-
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
73000
-
gel filtration, nonhomogenous preparation
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Cavia porcellus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Homo sapiens
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Rattus norvegicus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Sus scrofa
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Oryctolagus cuniculus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Canis lupus familiaris
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Macaca mulatta
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Rana sp.
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Columba sp.
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Felis catus domesticus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli B / ATCC 11303
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus BALB/c
-
uridine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.5
Canis lupus familiaris
-
dog
-
3.5.4.5
Cavia porcellus
-
guinea pig
-
3.5.4.5
Columba sp.
-
pigeon
-
3.5.4.5
Escherichia coli
-
-
-
3.5.4.5
Escherichia coli B / ATCC 11303
-
-
-
3.5.4.5
Felis catus domesticus
-
cat
-
3.5.4.5
Homo sapiens
-
HeLa cells; human
-
3.5.4.5
Macaca mulatta
-
rhesus monkey
-
3.5.4.5
Mus musculus
-
BALB/c; mouse
-
3.5.4.5
Mus musculus BALB/c
-
BALB/c
-
3.5.4.5
Oryctolagus cuniculus
-
rabbit
-
3.5.4.5
Rana sp.
-
frog
-
3.5.4.5
Rattus norvegicus
-
rat
-
3.5.4.5
Sus scrofa
-
pig
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.5
-
Escherichia coli
3.5.4.5
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.5
heart
-
Homo sapiens
-
3.5.4.5
kidney
-
Homo sapiens
-
3.5.4.5
kidney
-
Mus musculus
-
3.5.4.5
liver
-
Cavia porcellus
-
3.5.4.5
liver
-
Columba sp.
-
3.5.4.5
liver
-
Felis catus domesticus
-
3.5.4.5
liver
-
Oryctolagus cuniculus
-
3.5.4.5
liver
-
Rana sp.
-
3.5.4.5
liver
-
Rattus norvegicus
-
3.5.4.5
liver
-
Sus scrofa
-
3.5.4.5
liver
-
Macaca mulatta
-
3.5.4.5
liver
-
Canis lupus familiaris
-
3.5.4.5
liver
-
Mus musculus
-
3.5.4.5
liver
-
Homo sapiens
-
3.5.4.5
muscle
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.5
8.9
-
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.5
4°C, reasonably stable, retains 55% of its activity after 3 weeks
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
2'-deoxycytidine + H2O
-
209648
Mus musculus
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209648
Escherichia coli
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209648
Homo sapiens
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209648
Escherichia coli B / ATCC 11303
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209648
Mus musculus BALB/c
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-chlorocyclocytidine + H2O
i.e. cytosine, 1beta-D-arabinofuranosyl-2,2'-anhydro-, hydrochloride
209648
Homo sapiens
5'-chlorocyclouridine + NH3
-
-
-
?
3.5.4.5
5,6-dihydrocytidine + H2O
-
209648
Escherichia coli
5,6-dihydrouridine + NH3
-
-
-
-
3.5.4.5
5-azacytidine + H2O
-
209648
Escherichia coli
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209648
Homo sapiens
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-bromocytidine + H2O
-
209648
Homo sapiens
5-bromouridine + NH3
-
-
-
?
3.5.4.5
5-chlorocytidine + H2O
-
209648
Homo sapiens
? + NH3
-
-
-
?
3.5.4.5
5-iodocytidine + H2O
-
209648
Mus musculus
5-iodouridine + NH3
-
-
-
?
3.5.4.5
5-iodocytidine + H2O
-
209648
Homo sapiens
5-iodouridine + NH3
-
-
-
?
3.5.4.5
5-iodocytidine + H2O
-
209648
Mus musculus BALB/c
5-iodouridine + NH3
-
-
-
?
3.5.4.5
6-azacytidine + H2O
-
209648
Homo sapiens
6-azauridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Cavia porcellus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Mus musculus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Homo sapiens
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Rattus norvegicus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Sus scrofa
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Oryctolagus cuniculus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Canis lupus familiaris
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Macaca mulatta
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Rana sp.
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Columba sp.
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Felis catus domesticus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Escherichia coli B / ATCC 11303
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Mus musculus BALB/c
uridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209648
Mus musculus
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209648
Escherichia coli
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209648
Homo sapiens
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209648
Canis lupus familiaris
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209648
Escherichia coli B / ATCC 11303
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209648
Mus musculus BALB/c
deoxyuridine + NH3
-
-
-
?
3.5.4.5
additional information
-
209648
Escherichia coli
?
-
-
-
-
3.5.4.5
additional information
3-methylcytidine, cytosine and cyclocytidine are no substrates
209648
Homo sapiens
?
-
-
-
-
3.5.4.5
additional information
-
209648
Escherichia coli B / ATCC 11303
?
-
-
-
-
3.5.4.5
N4-methylcytidine + H2O
-
209648
Escherichia coli
uridine + methylamine
-
-
-
r
3.5.4.5
N4-methylcytidine + H2O
-
209648
Escherichia coli B / ATCC 11303
uridine + methylamine
-
-
-
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.5
5
-
identical rates of deamination of cytidine at pH 8 and pH 10
Homo sapiens
3.5.4.5
8
-
identical rates of deamination of cytidine at pH 5 and pH 10
Homo sapiens
3.5.4.5
10
-
identical rates of deamination of cytidine at pH 5 and pH 8
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.5
6.5
10.7
-
Escherichia coli
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.4.5
6.5
10.7
irreversible loss of activity at pH values below 4
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.5
5,6-Dihydrouridine
competitive inhibition
Escherichia coli
3.5.4.5
5,6-Dihydrouridine
-
Homo sapiens
3.5.4.5
uridine
-
Escherichia coli
3.5.4.5
uridine
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.0092
-
cytidine
-
Homo sapiens
3.5.4.5
0.058
-
5-azacytidine
-
Homo sapiens
3.5.4.5
0.089
-
cytosine deoxyriboside
-
Escherichia coli
3.5.4.5
0.11
-
5,6-Dihydrocytidine
-
Escherichia coli
3.5.4.5
0.14
-
cytosine arabinoside
-
Homo sapiens
3.5.4.5
0.21
-
cytidine
-
Escherichia coli
3.5.4.5
4.2
-
6-azacytidine
-
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
73000
-
gel filtration, nonhomogenous preparation
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
cytidine + H2O
Cavia porcellus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Homo sapiens
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Rattus norvegicus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Sus scrofa
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Oryctolagus cuniculus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Canis lupus familiaris
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Macaca mulatta
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Rana sp.
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Columba sp.
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Felis catus domesticus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli B / ATCC 11303
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus BALB/c
-
uridine + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
-
Escherichia coli
3.5.4.5
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.5
heart
-
Homo sapiens
-
3.5.4.5
kidney
-
Homo sapiens
-
3.5.4.5
kidney
-
Mus musculus
-
3.5.4.5
liver
-
Cavia porcellus
-
3.5.4.5
liver
-
Columba sp.
-
3.5.4.5
liver
-
Felis catus domesticus
-
3.5.4.5
liver
-
Oryctolagus cuniculus
-
3.5.4.5
liver
-
Rana sp.
-
3.5.4.5
liver
-
Rattus norvegicus
-
3.5.4.5
liver
-
Sus scrofa
-
3.5.4.5
liver
-
Macaca mulatta
-
3.5.4.5
liver
-
Canis lupus familiaris
-
3.5.4.5
liver
-
Mus musculus
-
3.5.4.5
liver
-
Homo sapiens
-
3.5.4.5
muscle
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.5
8.9
-
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.5
4°C, reasonably stable, retains 55% of its activity after 3 weeks
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
2'-deoxycytidine + H2O
-
209648
Mus musculus
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209648
Escherichia coli
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209648
Homo sapiens
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209648
Escherichia coli B / ATCC 11303
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209648
Mus musculus BALB/c
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
5'-chlorocyclocytidine + H2O
i.e. cytosine, 1beta-D-arabinofuranosyl-2,2'-anhydro-, hydrochloride
209648
Homo sapiens
5'-chlorocyclouridine + NH3
-
-
-
?
3.5.4.5
5,6-dihydrocytidine + H2O
-
209648
Escherichia coli
5,6-dihydrouridine + NH3
-
-
-
-
3.5.4.5
5-azacytidine + H2O
-
209648
Escherichia coli
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-azacytidine + H2O
-
209648
Homo sapiens
5-azauridine + NH3
-
-
-
?
3.5.4.5
5-bromocytidine + H2O
-
209648
Homo sapiens
5-bromouridine + NH3
-
-
-
?
3.5.4.5
5-chlorocytidine + H2O
-
209648
Homo sapiens
? + NH3
-
-
-
?
3.5.4.5
5-iodocytidine + H2O
-
209648
Mus musculus
5-iodouridine + NH3
-
-
-
?
3.5.4.5
5-iodocytidine + H2O
-
209648
Homo sapiens
5-iodouridine + NH3
-
-
-
?
3.5.4.5
5-iodocytidine + H2O
-
209648
Mus musculus BALB/c
5-iodouridine + NH3
-
-
-
?
3.5.4.5
6-azacytidine + H2O
-
209648
Homo sapiens
6-azauridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Cavia porcellus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Mus musculus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Homo sapiens
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Rattus norvegicus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Sus scrofa
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Oryctolagus cuniculus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Canis lupus familiaris
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Macaca mulatta
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Rana sp.
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Columba sp.
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Felis catus domesticus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Escherichia coli B / ATCC 11303
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209648
Mus musculus BALB/c
uridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209648
Mus musculus
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209648
Escherichia coli
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209648
Homo sapiens
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209648
Canis lupus familiaris
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209648
Escherichia coli B / ATCC 11303
deoxyuridine + NH3
-
-
-
?
3.5.4.5
deoxycytidine + H2O
-
209648
Mus musculus BALB/c
deoxyuridine + NH3
-
-
-
?
3.5.4.5
additional information
-
209648
Escherichia coli
?
-
-
-
-
3.5.4.5
additional information
3-methylcytidine, cytosine and cyclocytidine are no substrates
209648
Homo sapiens
?
-
-
-
-
3.5.4.5
additional information
-
209648
Escherichia coli B / ATCC 11303
?
-
-
-
-
3.5.4.5
N4-methylcytidine + H2O
-
209648
Escherichia coli
uridine + methylamine
-
-
-
r
3.5.4.5
N4-methylcytidine + H2O
-
209648
Escherichia coli B / ATCC 11303
uridine + methylamine
-
-
-
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.5
5
-
identical rates of deamination of cytidine at pH 8 and pH 10
Homo sapiens
3.5.4.5
8
-
identical rates of deamination of cytidine at pH 5 and pH 10
Homo sapiens
3.5.4.5
10
-
identical rates of deamination of cytidine at pH 5 and pH 8
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.5
6.5
10.7
-
Escherichia coli
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.4.5
6.5
10.7
irreversible loss of activity at pH values below 4
Escherichia coli