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Literature summary extracted from

  • Nygaard, P.
    Adenosine deaminase from Escherichia coli (1978), Methods Enzymol., 51, 508-512.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.4 coformycin
-
 Escherichia coli
3.5.4.4 p-chloromercuribenzoate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.4 0.04
-
 2'-deoxyadenosine
-
 Escherichia coli
3.5.4.4 0.075
-
 adenosine
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.4 29000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.4 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.4 277
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.5.4.4 4°C, Tris-HCl, pH 8.2, 20% ethylene glycol, 2 mM CaCl2, 50µM EDTA, several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.4 2',3'-dideoxyadenosine + H2O
-
 Escherichia coli 2',3'-dideoxyinosine + NH3
-
 ?
3.5.4.4 2'-deoxyadenosine + H2O
-
 Escherichia coli 2'-deoxyinosine + NH3
-
 ?
3.5.4.4 3'-deoxyadenosine + H2O
-
 Escherichia coli 3'-deoxyinosine + NH3
-
 ?
3.5.4.4 6-methylaminopurine ribonucleoside + H2O
-
 Escherichia coli inosine + methylamine
-
 ?
3.5.4.4 adenine + H2O
-
 Escherichia coli hypoxanthine + NH3
-
 ?
3.5.4.4 adenine arabinoside + H2O
-
 Escherichia coli inosine arabinoside + NH3
-
 ?
3.5.4.4 adenosine + H2O
-
 Escherichia coli inosine + NH3
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.4 6.9 8.5
-
 Escherichia coli