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Literature summary extracted from
- Cytosine deaminase that hydrolyzes creatinine to N-methylhydantoin in various cytosine deaminase-forming microorganisms (1987), Arch. Microbiol., 147, 58-63.
No PubMed abstract available
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.1 | 200000 | - |
- |
Proteus mirabilis |
| 3.5.4.1 | 200000 | - |
gel filtration | Escherichia coli |
| 3.5.4.1 | 260000 | - |
- |
Pseudomonas putida |
| 3.5.4.1 | 300000 | - |
gel filtration | Escherichia coli |
| 3.5.4.21 | 260000 | - |
gel filtration | Pseudomonas putida 77 |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.1 | cytosine + H2O | Escherichia coli | - |
uracil + NH3 | - |
? |  |
| 3.5.4.1 | cytosine + H2O | Pseudomonas putida | - |
uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | Proteus mirabilis | - |
uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | Pseudomonas chlororaphis | - |
uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | Pseudomonas sp. 'cruciviae' | - |
uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | Pseudomonas chlororaphis subsp. aureofaciens | - |
uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | Flavobacterium filamentosum | - |
uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | Escherichia coli K12 AKU 0005 | - |
uracil + NH3 | - |
? | |
| 3.5.4.21 | creatinine + H2O | Escherichia coli | - |
N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.21 | creatinine + H2O | Proteus mirabilis | - |
N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.21 | creatinine + H2O | Pseudomonas chlororaphis | - |
N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.21 | creatinine + H2O | Pseudomonas sp. 'cruciviae' | - |
N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.21 | creatinine + H2O | Pseudomonas chlororaphis subsp. aureofaciens | - |
N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.21 | creatinine + H2O | Pseudomonas putida 77 | - |
N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.21 | creatinine + H2O | Escherichia coli K12 AKU 0005 | - |
N-methylhydantoin + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.1 | Escherichia coli | - |
- |
- |
| 3.5.4.1 | Escherichia coli K12 AKU 0005 | - |
- |
- |
| 3.5.4.1 | Flavobacterium filamentosum | - |
- |
- |
| 3.5.4.1 | Proteus mirabilis | - |
IFO 3849 | - |
| 3.5.4.1 | Pseudomonas chlororaphis | - |
IFO 3904 | - |
| 3.5.4.1 | Pseudomonas chlororaphis subsp. aureofaciens | - |
IFO 3521 | - |
| 3.5.4.1 | Pseudomonas putida | - |
IFO 3738 | - |
| 3.5.4.1 | Pseudomonas putida | - |
77 AKU 875 | - |
| 3.5.4.1 | Pseudomonas sp. 'cruciviae' | - |
IFO 12047 | - |
| 3.5.4.21 | Escherichia coli | - |
- |
- |
| 3.5.4.21 | Proteus mirabilis | - |
- |
- |
| 3.5.4.21 | Pseudomonas chlororaphis | - |
- |
- |
| 3.5.4.21 | Pseudomonas chlororaphis subsp. aureofaciens | - |
- |
- |
| 3.5.4.21 | Pseudomonas putida 77 | - |
- |
- |
| 3.5.4.21 | Pseudomonas sp. 'cruciviae' | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.21 | 0.0004 | - |
cytosine added to culture medium | Pseudomonas sp. 'cruciviae' |
| 3.5.4.21 | 0.0011 | - |
cytosine added to culture medium | Proteus mirabilis |
| 3.5.4.21 | 0.0013 | - |
cytosine added to culture medium | Pseudomonas chlororaphis subsp. aureofaciens |
| 3.5.4.21 | 0.0014 | - |
polypeptone added to culture medium | Pseudomonas sp. 'cruciviae' |
| 3.5.4.21 | 0.0015 | - |
NH4Cl added to culture medium | Pseudomonas chlororaphis subsp. aureofaciens |
| 3.5.4.21 | 0.0016 | - |
NH4Cl added to culture medium | Proteus mirabilis |
| 3.5.4.21 | 0.0018 | - |
polypeptone added to culture medium | Escherichia coli |
| 3.5.4.21 | 0.0021 | - |
NH4Cl added to culture medium | Escherichia coli |
| 3.5.4.21 | 0.0024 | - |
cytosine added to culture medium | Escherichia coli |
| 3.5.4.21 | 0.0034 | - |
NH4Cl added to culture medium | Pseudomonas chlororaphis |
| 3.5.4.21 | 0.0051 | - |
creatinine added to culture medium | Proteus mirabilis |
| 3.5.4.21 | 0.0056 | - |
polypeptone added to culture medium | Pseudomonas chlororaphis subsp. aureofaciens |
| 3.5.4.21 | 0.0062 | - |
polypeptone added to culture medium | Proteus mirabilis |
| 3.5.4.21 | 0.0064 | - |
NH4Cl added to culture medium | Pseudomonas putida 77 |
| 3.5.4.21 | 0.0065 | - |
polypeptone added to culture medium | Pseudomonas chlororaphis |
| 3.5.4.21 | 0.0072 | - |
polypeptone added to culture medium | Pseudomonas putida 77 |
| 3.5.4.21 | 0.0092 | - |
cytosine added to culture medium | Pseudomonas chlororaphis |
| 3.5.4.21 | 0.01 | - |
creatinine added to culture medium | Pseudomonas chlororaphis subsp. aureofaciens |
| 3.5.4.21 | 0.0112 | - |
creatinine added to culture medium | Pseudomonas chlororaphis |
| 3.5.4.21 | 0.0216 | - |
creatinine added to culture medium | Escherichia coli |
| 3.5.4.21 | 0.0729 | - |
creatinine added to culture medium | Pseudomonas putida 77 |
| 3.5.4.21 | 0.089 | - |
cytosine added to culture medium | Pseudomonas putida 77 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.1 | creatinine + H2O | - |
Flavobacterium filamentosum | N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.1 | creatinine + H2O | - |
Escherichia coli | N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.1 | creatinine + H2O | - |
Pseudomonas putida | N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.1 | creatinine + H2O | - |
Proteus mirabilis | N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.1 | creatinine + H2O | - |
Pseudomonas chlororaphis | N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.1 | creatinine + H2O | - |
Pseudomonas sp. 'cruciviae' | N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.1 | creatinine + H2O | - |
Pseudomonas chlororaphis subsp. aureofaciens | N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.1 | creatinine + H2O | - |
Escherichia coli K12 AKU 0005 | N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | - |
Pseudomonas putida | uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | - |
Proteus mirabilis | uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | - |
Pseudomonas chlororaphis | uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | - |
Pseudomonas sp. 'cruciviae' | uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | - |
Pseudomonas chlororaphis subsp. aureofaciens | uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | - |
Flavobacterium filamentosum | uracil + NH3 | - |
? | |
| 3.5.4.1 | cytosine + H2O | - |
Escherichia coli K12 AKU 0005 | uracil + NH3 | - |
? | |
| 3.5.4.1 | additional information | creatinine is no substrate | Pseudomonas putida | ? | - |
? | |
| 3.5.4.21 | creatinine + H2O | - |
Escherichia coli | N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.21 | creatinine + H2O | - |
Proteus mirabilis | N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.21 | creatinine + H2O | - |
Pseudomonas chlororaphis | N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.21 | creatinine + H2O | - |
Pseudomonas sp. 'cruciviae' | N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.21 | creatinine + H2O | - |
Pseudomonas chlororaphis subsp. aureofaciens | N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.21 | creatinine + H2O | - |
Pseudomonas putida 77 | N-methylhydantoin + NH3 | - |
? | |
| 3.5.4.21 | creatinine + H2O | - |
Escherichia coli K12 AKU 0005 | N-methylhydantoin + NH3 | - |
? | |
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