BRENDA - Enzyme Database show

Cytosine and cytidine deaminase from yeast

Ipata, P.L.; Cercignani, G.; Methods Enzymol. 51, 394-401 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.1
CDP
-
Saccharomyces cerevisiae
3.5.4.1
CMP
-
Saccharomyces cerevisiae
3.5.4.1
CTP
-
Saccharomyces cerevisiae
3.5.4.1
cytidine
-
Saccharomyces cerevisiae
3.5.4.1
dTTP
-
Saccharomyces cerevisiae
3.5.4.1
GDP
-
Saccharomyces cerevisiae
3.5.4.1
GMP
-
Saccharomyces cerevisiae
3.5.4.1
GTP
-
Saccharomyces cerevisiae
3.5.4.1
guanosine
-
Saccharomyces cerevisiae
3.5.4.1
thymidine
-
Saccharomyces cerevisiae
3.5.4.5
CDP
-
Saccharomyces cerevisiae
3.5.4.5
CMP
0.300 mM 50% inhibition, 0.750 mM 90% inhibition
Saccharomyces cerevisiae
3.5.4.5
CTP
-
Saccharomyces cerevisiae
3.5.4.5
cytidine
strong substrate inhibition above 0.5 mM
Saccharomyces cerevisiae
3.5.4.5
dTMP
0.400 mM 50% inhibition
Saccharomyces cerevisiae
3.5.4.5
dTTP
0.400 mM 50% inhibition
Saccharomyces cerevisiae
3.5.4.5
dTTP
-
Mus musculus
3.5.4.5
GTP
0.750 mM 85% inhibition
Saccharomyces cerevisiae
3.5.4.5
p-mercuribenzoate
highly sensitive
Saccharomyces cerevisiae
3.5.4.5
p-mercuribenzoate
highly sensitive
Mus musculus
3.5.4.5
UTP
-
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.1
2.5
-
5-Methylcytosine
-
Saccharomyces cerevisiae
3.5.4.1
2.5
-
cytosine
-
Saccharomyces cerevisiae
3.5.4.5
0.091
-
2-deoxycytidine
-
Saccharomyces cerevisiae
3.5.4.5
0.25
-
cytidine
-
Escherichia coli
3.5.4.5
0.25
-
cytidine
-
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.1
34000
-
-
Saccharomyces cerevisiae
3.5.4.5
57000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.1
cytosine + H2O
Saccharomyces cerevisiae
-
uracil + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Saccharomyces cerevisiae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus BALB/c
-
uridine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.1
Saccharomyces cerevisiae
-
-
-
3.5.4.5
Escherichia coli
-
-
-
3.5.4.5
Mus musculus
-
BALB/c; mouse
-
3.5.4.5
Mus musculus BALB/c
-
BALB/c
-
3.5.4.5
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.1
-
Saccharomyces cerevisiae
3.5.4.5
-
Saccharomyces cerevisiae
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.5
kidney
-
Mus musculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.5
3.128
-
-
Saccharomyces cerevisiae
Storage Stability
EC Number
Storage Stability
Organism
3.5.4.1
4°C, stable in the pH-range 5-9 for at least 48 h
Saccharomyces cerevisiae
3.5.4.5
-20°C, shows no appreciable loss of activity within 1 month
Saccharomyces cerevisiae
3.5.4.5
4°C, stable for some hours at pH 5.5-9.5
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.1
5-methylcytosine + H2O
-
209492
Saccharomyces cerevisiae
thymine + NH3
-
-
-
-
3.5.4.1
cytosine + H2O
-
209492
Saccharomyces cerevisiae
uracil + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209492
Mus musculus
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209492
Escherichia coli
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209492
Saccharomyces cerevisiae
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209492
Mus musculus BALB/c
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209492
Mus musculus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209492
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209492
Saccharomyces cerevisiae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209492
Mus musculus BALB/c
uridine + NH3
-
-
-
?
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.4.5
40
62
heat treatment for 5 min at 50-60°C completely abolishes CMP and substrate inhibition
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.1
6.5
-
-
Saccharomyces cerevisiae
3.5.4.5
7.2
-
-
Mus musculus
3.5.4.5
7.2
-
-
Saccharomyces cerevisiae
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.4.5
5.5
9.5
stable for some hours at 4°C, rapid inactivation and precipitation of the enzyme occurs at pH lower 5
Saccharomyces cerevisiae
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.4.5
0.3
-
CMP
-
Saccharomyces cerevisiae
3.5.4.5
0.4
-
dTMP
-
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.1
CDP
-
Saccharomyces cerevisiae
3.5.4.1
CMP
-
Saccharomyces cerevisiae
3.5.4.1
CTP
-
Saccharomyces cerevisiae
3.5.4.1
cytidine
-
Saccharomyces cerevisiae
3.5.4.1
dTTP
-
Saccharomyces cerevisiae
3.5.4.1
GDP
-
Saccharomyces cerevisiae
3.5.4.1
GMP
-
Saccharomyces cerevisiae
3.5.4.1
GTP
-
Saccharomyces cerevisiae
3.5.4.1
guanosine
-
Saccharomyces cerevisiae
3.5.4.1
thymidine
-
Saccharomyces cerevisiae
3.5.4.5
CDP
-
Saccharomyces cerevisiae
3.5.4.5
CMP
0.300 mM 50% inhibition, 0.750 mM 90% inhibition
Saccharomyces cerevisiae
3.5.4.5
CTP
-
Saccharomyces cerevisiae
3.5.4.5
cytidine
strong substrate inhibition above 0.5 mM
Saccharomyces cerevisiae
3.5.4.5
dTMP
0.400 mM 50% inhibition
Saccharomyces cerevisiae
3.5.4.5
dTTP
0.400 mM 50% inhibition
Saccharomyces cerevisiae
3.5.4.5
dTTP
-
Mus musculus
3.5.4.5
GTP
0.750 mM 85% inhibition
Saccharomyces cerevisiae
3.5.4.5
p-mercuribenzoate
highly sensitive
Saccharomyces cerevisiae
3.5.4.5
p-mercuribenzoate
highly sensitive
Mus musculus
3.5.4.5
UTP
-
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.4.5
0.3
-
CMP
-
Saccharomyces cerevisiae
3.5.4.5
0.4
-
dTMP
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.1
2.5
-
5-Methylcytosine
-
Saccharomyces cerevisiae
3.5.4.1
2.5
-
cytosine
-
Saccharomyces cerevisiae
3.5.4.5
0.091
-
2-deoxycytidine
-
Saccharomyces cerevisiae
3.5.4.5
0.25
-
cytidine
-
Escherichia coli
3.5.4.5
0.25
-
cytidine
-
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.1
34000
-
-
Saccharomyces cerevisiae
3.5.4.5
57000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.1
cytosine + H2O
Saccharomyces cerevisiae
-
uracil + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Escherichia coli
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Saccharomyces cerevisiae
-
uridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Mus musculus BALB/c
-
uridine + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.1
-
Saccharomyces cerevisiae
3.5.4.5
-
Saccharomyces cerevisiae
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.4.5
kidney
-
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.4.5
3.128
-
-
Saccharomyces cerevisiae
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.4.1
4°C, stable in the pH-range 5-9 for at least 48 h
Saccharomyces cerevisiae
3.5.4.5
-20°C, shows no appreciable loss of activity within 1 month
Saccharomyces cerevisiae
3.5.4.5
4°C, stable for some hours at pH 5.5-9.5
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.1
5-methylcytosine + H2O
-
209492
Saccharomyces cerevisiae
thymine + NH3
-
-
-
-
3.5.4.1
cytosine + H2O
-
209492
Saccharomyces cerevisiae
uracil + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209492
Mus musculus
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209492
Escherichia coli
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209492
Saccharomyces cerevisiae
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
2'-deoxycytidine + H2O
-
209492
Mus musculus BALB/c
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209492
Mus musculus
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209492
Escherichia coli
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209492
Saccharomyces cerevisiae
uridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
209492
Mus musculus BALB/c
uridine + NH3
-
-
-
?
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.4.5
40
62
heat treatment for 5 min at 50-60°C completely abolishes CMP and substrate inhibition
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.1
6.5
-
-
Saccharomyces cerevisiae
3.5.4.5
7.2
-
-
Mus musculus
3.5.4.5
7.2
-
-
Saccharomyces cerevisiae
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.4.5
5.5
9.5
stable for some hours at 4°C, rapid inactivation and precipitation of the enzyme occurs at pH lower 5
Saccharomyces cerevisiae