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Literature summary extracted from

  • Sakai, T.; Yu, T.; Tabe, H.; Omata, S.
    Purification of cytosine deaminase from Serratia marcescens (1975), Agric. Biol. Chem., 39, 1623-1629.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.1 ATP
-
 Serratia marcescens
3.5.4.1 phosphate
-
 Serratia marcescens

General Stability

EC Number General Stability Organism
3.5.4.1 stable in cell-free extracts Serratia marcescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.1 34000
-
-
 Saccharomyces cerevisiae
3.5.4.1 72000
-
 8 * 72000, SDS-PAGE Serratia marcescens
3.5.4.1 580000
-
 gel filtration Serratia marcescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.1 cytosine + H2O Escherichia coli
-
 uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O Saccharomyces cerevisiae
-
 uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O Serratia marcescens
-
 uracil + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.1 Escherichia coli
-
-
-
3.5.4.1 Saccharomyces cerevisiae
-
 baker's yeast
-
3.5.4.1 Serratia marcescens
-
 IFO 3054
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.1
-
 Serratia marcescens
3.5.4.1 partially Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.1 1.2
-
-
 Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.1 5-methylcytosine + H2O
-
 Saccharomyces cerevisiae thymine + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Escherichia coli uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Saccharomyces cerevisiae uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Serratia marcescens uracil + NH3
-
 ?
3.5.4.1 additional information 5-methylcytosine, cytidine, 5'-CMP, adenine, adenosine, 5'-AMP, guanine, guanosine, 5'-GMP and GMP are no substrates Serratia marcescens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.1 octamer 8 * 72000, SDS-PAGE Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.1 70
-
 after treating for 10 min at pH 7, 75% activity remains Serratia marcescens