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Literature summary extracted from

  • Takaku, H.; Takase, M.; Abe, S.i.; Hayashi, H.; Miyazaki, K.
    In vivo anti-tumor activity of arginine deiminase purified from Mycoplasma arginini (1992), Int. J. Cancer, 51, 244-249.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.6 0.2
-
 L-Arg
-
 Pseudomonas putida
3.5.3.6 0.2
-
 L-Arg
-
 Mycoplasmopsis arginini

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.6 45000
-
 2 * 45000, SDS-PAGE Pseudomonas putida
3.5.3.6 45000
-
 2 * 45000, SDS-PAGE Mycoplasmopsis arginini
3.5.3.6 90000
-
 gel filtration Mycoplasmopsis arginini

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.6 L-Arg + H2O Mycoplasmopsis arginini in vivo and in vitro anti-tumor activities for 6 kinds of mouse ascites tumor cell lines citrulline + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.6 Mycoplasmopsis arginini
-
-
-
3.5.3.6 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.6
-
 Mycoplasmopsis arginini

Storage Stability

EC Number Storage Stability Organism
3.5.3.6 4°C, pH 7.0, stable for more than 1 month Mycoplasmopsis arginini

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.6 L-Arg + H2O
-
 Pseudomonas putida L-citrulline + NH3
-
 ?
3.5.3.6 L-Arg + H2O
-
 Mycoplasmopsis arginini L-citrulline + NH3
-
 ?
3.5.3.6 L-Arg + H2O in vivo and in vitro anti-tumor activities for 6 kinds of mouse ascites tumor cell lines Mycoplasmopsis arginini citrulline + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.6 dimer 2 * 45000, SDS-PAGE Pseudomonas putida
3.5.3.6 dimer 2 * 45000, SDS-PAGE Mycoplasmopsis arginini

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.6 50
-
-
 Pseudomonas putida
3.5.3.6 50
-
-
 Mycoplasmopsis arginini

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.6 6 7.5
-
 Mycoplasmopsis arginini
3.5.3.6 6
-
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.6 6 7.4 pH 6.0: maximal activity, pH 7.4: 13% of maximal activity Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.3.6 7
-
 4°C, stable for more than 1 months Mycoplasmopsis arginini