BRENDA - Enzyme Database

Cloning and expression of a prokaryotic enzyme, arginine deiminase, from primitive eukaryote Giardia intestinalis

Knodler, L.A.; Sekyere, E.O.; Stewart, T.S.; Schofield, P.J.; Edwards, M.R.; J. Biol. Chem. 273, 4470-4477 (1998)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.3.6
expression in Escherichia coli
Giardia intestinalis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.6
agmatine
-
Giardia intestinalis
3.5.3.6
L-canavanine
-
Giardia intestinalis
3.5.3.6
L-homoarginine
-
Giardia intestinalis
3.5.3.6
L-ornithine
-
Giardia intestinalis
3.5.3.6
putrescine
-
Giardia intestinalis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.6
66000
-
2 * 66000, SDS-PAGE
Giardia intestinalis
3.5.3.6
140000
-
gel filtration
Giardia intestinalis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.6
L-Arg + H2O
Giardia intestinalis
enzyme of catabolism of Arg to citrulline in the arginine dihydrolase pathway
citrulline + NH3
-
Giardia intestinalis
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.6
Giardia intestinalis
Q27657
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.6
recombinant enzyme
Giardia intestinalis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.3.6
36.5
-
recombinant enzyme
Giardia intestinalis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.6
L-Arg + H2O
-
209460
Giardia intestinalis
L-citrulline + NH3
-
209460
Giardia intestinalis
ir
3.5.3.6
L-Arg + H2O
enzyme of catabolism of Arg to citrulline in the arginine dihydrolase pathway
209460
Giardia intestinalis
citrulline + NH3
-
209460
Giardia intestinalis
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.3.6
dimer
2 * 66000, SDS-PAGE
Giardia intestinalis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.6
expression in Escherichia coli
Giardia intestinalis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.6
agmatine
-
Giardia intestinalis
3.5.3.6
L-canavanine
-
Giardia intestinalis
3.5.3.6
L-homoarginine
-
Giardia intestinalis
3.5.3.6
L-ornithine
-
Giardia intestinalis
3.5.3.6
putrescine
-
Giardia intestinalis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.6
66000
-
2 * 66000, SDS-PAGE
Giardia intestinalis
3.5.3.6
140000
-
gel filtration
Giardia intestinalis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.6
L-Arg + H2O
Giardia intestinalis
enzyme of catabolism of Arg to citrulline in the arginine dihydrolase pathway
citrulline + NH3
-
Giardia intestinalis
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.6
recombinant enzyme
Giardia intestinalis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.3.6
36.5
-
recombinant enzyme
Giardia intestinalis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.6
L-Arg + H2O
-
209460
Giardia intestinalis
L-citrulline + NH3
-
209460
Giardia intestinalis
ir
3.5.3.6
L-Arg + H2O
enzyme of catabolism of Arg to citrulline in the arginine dihydrolase pathway
209460
Giardia intestinalis
citrulline + NH3
-
209460
Giardia intestinalis
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.3.6
dimer
2 * 66000, SDS-PAGE
Giardia intestinalis