BRENDA - Enzyme Database

Diguanidinobutanase of Pseudomonas putida and enzymatic preparation of agmatine from 1,4-diguanidinobutanate

Shoeb, S.M.; Kaneoke, M.; Shimizu, E.; Yorifuji, T.; Appl. Microbiol. Biotechnol. 44, 94-99 (1995)
No PubMed abstract available

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.5.3.20
synthesis
preparation of pure agmatine sulfate
Pseudomonas putida
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.20
1,10-Diaminodecane
41% inhibition at concentration of 3.0 mM
Pseudomonas putida
3.5.3.20
1,7-Diaminoheptane
22% inhibition at concentration of 5.0 mM
Pseudomonas putida
3.5.3.20
1,8-diaminooctane
34% inhibition at concentration of 3.0 mM
Pseudomonas putida
3.5.3.20
1,9-diaminononane
38% inhibition at concentration of 3.0 mM
Pseudomonas putida
3.5.3.20
1-amino-5-guanidinopentane
29% inhibition at concentration of 5.0 mM
Pseudomonas putida
3.5.3.20
5-Guanidinovalerate
10% inhibition at concentration of 5.0 mM
Pseudomonas putida
3.5.3.20
agmatine
28% inhibition at concentration of 5.0 mM
Pseudomonas putida
3.5.3.20
DTNB
11% inhibition at concentration of 5.0 mM
Pseudomonas putida
3.5.3.20
N-carbamoylagmatine
62% inhibition at concentration of 5.0 mM
Pseudomonas putida
3.5.3.20
p-chloromercuribenzoate
complete loss of activity at concentration of 5.0 mM, 30% reactivation by incubation with 10.0 mM 2-mercaptoethanol
Pseudomonas putida
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.20
0.23
-
1,8-Diguanidinooctane
-
Pseudomonas putida
3.5.3.20
0.24
-
1,7-Diguanidinoheptane
-
Pseudomonas putida
3.5.3.20
0.33
-
1,6-Diguanidinohexane
-
Pseudomonas putida
3.5.3.20
0.5
-
1,5-Diguanidinopentane
-
Pseudomonas putida
3.5.3.20
0.65
-
1,4-Diguanidinobutane
-
Pseudomonas putida
3.5.3.20
0.91
-
1,3-diguanidinopropane
-
Pseudomonas putida
3.5.3.20
1
-
1-amino-7-guanidinoheptane
-
Pseudomonas putida
3.5.3.20
1.3
-
1-amino-6-guanidinohexane
-
Pseudomonas putida
3.5.3.20
2.5
-
1-amino-5-guanidinopentane
-
Pseudomonas putida
3.5.3.20
3
-
agmatine
-
Pseudomonas putida
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.3.20
Ca2+
inert
Pseudomonas putida
3.5.3.20
Co2+
required for full activity
Pseudomonas putida
3.5.3.20
Mg2+
inert
Pseudomonas putida
3.5.3.20
Mn2+
required for full activity, no inactivation by incubation with 10 mM EDTA; two ions per subunit
Pseudomonas putida
3.5.3.20
Ni2+
required for full activity
Pseudomonas putida
3.5.3.20
Zn2+
inert
Pseudomonas putida
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.20
170000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.20
1,4-diguanidinobutane + H2O
Pseudomonas putida
-
agmatine + urea
-
Pseudomonas putida
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.20
Pseudomonas putida
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.20
full purification
Pseudomonas putida
Storage Stability
EC Number
Storage Stability
Organism
3.5.3.20
pH 7.0-8.0
Pseudomonas putida
3.5.3.20
pH 7.0-9.0
Pseudomonas putida
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.20
1,4-diguanidinobutane + H2O
-
209444
Pseudomonas putida
agmatine + urea
-
209444
Pseudomonas putida
?
3.5.3.20
1-amino-5-guanidinopentane + H2O
-
209444
Pseudomonas putida
1,5-diaminopentane + urea
-
-
-
?
3.5.3.20
1-amino-6-guanidinohexane + H2O
-
209444
Pseudomonas putida
1,6-diaminohexane + urea
-
-
-
?
3.5.3.20
1-amino-7-guanidinoheptane + H2O
-
209444
Pseudomonas putida
1,7-diaminoheptane + urea
-
-
-
?
3.5.3.20
agmatine + H2O
low activity towards agmatine
209444
Pseudomonas putida
putrescine + urea
-
-
-
?
3.5.3.20
diguanidinoalkanoate + H2O
utilization of alkanoates with 3-10 methylen groups
209444
Pseudomonas putida
aminoalkylguanidine + urea
-
209444
Pseudomonas putida
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.3.20
tetramer
4*38000 Da, SDS-PAGE
Pseudomonas putida
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.3.20
30
-
no loss of activity within 2 h in presence of 10 mM EDTA
Pseudomonas putida
3.5.3.20
40
-
stable on heating in presence of Mn2+
Pseudomonas putida
3.5.3.20
60
-
complete inactivation in presence and absence of Mn2+
Pseudomonas putida
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.3.20
1330
-
1,4-Diguanidinobutane
per subunit
Pseudomonas putida
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.20
10
11
in sodium carbonate and piperazine hydrochloric buffers
Pseudomonas putida
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.3.20
7
8
stable in potassium phosphate
Pseudomonas putida
3.5.3.20
7
9
stable in TRIS-HCl
Pseudomonas putida
3.5.3.20
10
-
stable in piperazine hydrochloride
Pseudomonas putida
Application (protein specific)
EC Number
Application
Commentary
Organism
3.5.3.20
synthesis
preparation of pure agmatine sulfate
Pseudomonas putida
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.20
1,10-Diaminodecane
41% inhibition at concentration of 3.0 mM
Pseudomonas putida
3.5.3.20
1,7-Diaminoheptane
22% inhibition at concentration of 5.0 mM
Pseudomonas putida
3.5.3.20
1,8-diaminooctane
34% inhibition at concentration of 3.0 mM
Pseudomonas putida
3.5.3.20
1,9-diaminononane
38% inhibition at concentration of 3.0 mM
Pseudomonas putida
3.5.3.20
1-amino-5-guanidinopentane
29% inhibition at concentration of 5.0 mM
Pseudomonas putida
3.5.3.20
5-Guanidinovalerate
10% inhibition at concentration of 5.0 mM
Pseudomonas putida
3.5.3.20
agmatine
28% inhibition at concentration of 5.0 mM
Pseudomonas putida
3.5.3.20
DTNB
11% inhibition at concentration of 5.0 mM
Pseudomonas putida
3.5.3.20
N-carbamoylagmatine
62% inhibition at concentration of 5.0 mM
Pseudomonas putida
3.5.3.20
p-chloromercuribenzoate
complete loss of activity at concentration of 5.0 mM, 30% reactivation by incubation with 10.0 mM 2-mercaptoethanol
Pseudomonas putida
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.20
0.23
-
1,8-Diguanidinooctane
-
Pseudomonas putida
3.5.3.20
0.24
-
1,7-Diguanidinoheptane
-
Pseudomonas putida
3.5.3.20
0.33
-
1,6-Diguanidinohexane
-
Pseudomonas putida
3.5.3.20
0.5
-
1,5-Diguanidinopentane
-
Pseudomonas putida
3.5.3.20
0.65
-
1,4-Diguanidinobutane
-
Pseudomonas putida
3.5.3.20
0.91
-
1,3-diguanidinopropane
-
Pseudomonas putida
3.5.3.20
1
-
1-amino-7-guanidinoheptane
-
Pseudomonas putida
3.5.3.20
1.3
-
1-amino-6-guanidinohexane
-
Pseudomonas putida
3.5.3.20
2.5
-
1-amino-5-guanidinopentane
-
Pseudomonas putida
3.5.3.20
3
-
agmatine
-
Pseudomonas putida
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.3.20
Ca2+
inert
Pseudomonas putida
3.5.3.20
Co2+
required for full activity
Pseudomonas putida
3.5.3.20
Mg2+
inert
Pseudomonas putida
3.5.3.20
Mn2+
required for full activity, no inactivation by incubation with 10 mM EDTA; two ions per subunit
Pseudomonas putida
3.5.3.20
Ni2+
required for full activity
Pseudomonas putida
3.5.3.20
Zn2+
inert
Pseudomonas putida
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.20
170000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.20
1,4-diguanidinobutane + H2O
Pseudomonas putida
-
agmatine + urea
-
Pseudomonas putida
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.20
full purification
Pseudomonas putida
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.3.20
pH 7.0-8.0
Pseudomonas putida
3.5.3.20
pH 7.0-9.0
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.20
1,4-diguanidinobutane + H2O
-
209444
Pseudomonas putida
agmatine + urea
-
209444
Pseudomonas putida
?
3.5.3.20
1-amino-5-guanidinopentane + H2O
-
209444
Pseudomonas putida
1,5-diaminopentane + urea
-
-
-
?
3.5.3.20
1-amino-6-guanidinohexane + H2O
-
209444
Pseudomonas putida
1,6-diaminohexane + urea
-
-
-
?
3.5.3.20
1-amino-7-guanidinoheptane + H2O
-
209444
Pseudomonas putida
1,7-diaminoheptane + urea
-
-
-
?
3.5.3.20
agmatine + H2O
low activity towards agmatine
209444
Pseudomonas putida
putrescine + urea
-
-
-
?
3.5.3.20
diguanidinoalkanoate + H2O
utilization of alkanoates with 3-10 methylen groups
209444
Pseudomonas putida
aminoalkylguanidine + urea
-
209444
Pseudomonas putida
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.3.20
tetramer
4*38000 Da, SDS-PAGE
Pseudomonas putida
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.3.20
30
-
no loss of activity within 2 h in presence of 10 mM EDTA
Pseudomonas putida
3.5.3.20
40
-
stable on heating in presence of Mn2+
Pseudomonas putida
3.5.3.20
60
-
complete inactivation in presence and absence of Mn2+
Pseudomonas putida
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.3.20
1330
-
1,4-Diguanidinobutane
per subunit
Pseudomonas putida
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.20
10
11
in sodium carbonate and piperazine hydrochloric buffers
Pseudomonas putida
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.3.20
7
8
stable in potassium phosphate
Pseudomonas putida
3.5.3.20
7
9
stable in TRIS-HCl
Pseudomonas putida
3.5.3.20
10
-
stable in piperazine hydrochloride
Pseudomonas putida