BRENDA - Enzyme Database

Degradation of alpha,omega-diguanidinoalkanoates and a novel enzyme, diguanidinobutane amidohydrolase in Pseudomonas putida

Yorifuji, T.; Kaneoke, M.; Shimizu, E.; Shiota, K.; Matsuo, R.; Agric. Biol. Chem. 53, 3003-3009 (1989)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.20
0.7
-
1,4-Diguanidinobutane
-
Pseudomonas putida
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.3.20
Co2+
required for full activity
Pseudomonas putida
3.5.3.20
Mn2+
required for full activity, no inactivation by incubation with 10 mM EDTA
Pseudomonas putida
3.5.3.20
Ni2+
required for full activity
Pseudomonas putida
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.20
1,4-diguanidinobutane + H2O
Pseudomonas putida
-
agmatine + urea
-
Pseudomonas putida
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.20
Pseudomonas putida
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.20
partial purification
Pseudomonas putida
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.20
1,4-diguanidinobutane + H2O
-
209443
Pseudomonas putida
agmatine + urea
-
209443
Pseudomonas putida
?
3.5.3.20
agmatine + H2O
low activity towards agmatine
209443
Pseudomonas putida
putrescine + urea
-
209443
Pseudomonas putida
?
3.5.3.20
diguanidinoalkanoate + H2O
utilization of alkanoates with 3-10 methylen groups
209443
Pseudomonas putida
aminoalkylguanidine + urea
-
209443
Pseudomonas putida
?
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.5.3.20
40
-
stable on heating for 15 min
Pseudomonas putida
3.5.3.20
50
-
unstable on heating for 15 min
Pseudomonas putida
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.20
10
11
-
Pseudomonas putida
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.3.20
7
10
most stable
Pseudomonas putida
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.20
0.7
-
1,4-Diguanidinobutane
-
Pseudomonas putida
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.3.20
Co2+
required for full activity
Pseudomonas putida
3.5.3.20
Mn2+
required for full activity, no inactivation by incubation with 10 mM EDTA
Pseudomonas putida
3.5.3.20
Ni2+
required for full activity
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.20
1,4-diguanidinobutane + H2O
Pseudomonas putida
-
agmatine + urea
-
Pseudomonas putida
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.20
partial purification
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.20
1,4-diguanidinobutane + H2O
-
209443
Pseudomonas putida
agmatine + urea
-
209443
Pseudomonas putida
?
3.5.3.20
agmatine + H2O
low activity towards agmatine
209443
Pseudomonas putida
putrescine + urea
-
209443
Pseudomonas putida
?
3.5.3.20
diguanidinoalkanoate + H2O
utilization of alkanoates with 3-10 methylen groups
209443
Pseudomonas putida
aminoalkylguanidine + urea
-
209443
Pseudomonas putida
?
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.5.3.20
40
-
stable on heating for 15 min
Pseudomonas putida
3.5.3.20
50
-
unstable on heating for 15 min
Pseudomonas putida
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.20
10
11
-
Pseudomonas putida
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.5.3.20
7
10
most stable
Pseudomonas putida