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Literature summary extracted from

  • Zygmunt, D.J.; Stratton, C.W.; Kernodle, D.S.
    Characterization of four beta-lactamases produced by Straphylococcus aureus (1992), Antimicrob. Agents Chemother., 36, 440-445.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.0032
-
 cefamandole beta-lactamase D Staphylococcus aureus
3.5.2.6 0.0047
-
 cephalothin beta-lactamase D Staphylococcus aureus
3.5.2.6 0.005
-
 cefamandole beta-lactamase A Staphylococcus aureus
3.5.2.6 0.0052
-
 cephapirin beta-lactamase A Staphylococcus aureus
3.5.2.6 0.0052
-
 cephalothin beta-lactamase C Staphylococcus aureus
3.5.2.6 0.0059
-
 nitrocefin beta-lactamase D Staphylococcus aureus
3.5.2.6 0.006
-
 cefamandole beta-lactamase B Staphylococcus aureus
3.5.2.6 0.0062
-
 cephalothin beta-lactamase B Staphylococcus aureus
3.5.2.6 0.0064
-
 nitrocefin beta-lactamase A Staphylococcus aureus
3.5.2.6 0.0065
-
 cefamandole beta-lactamase C Staphylococcus aureus
3.5.2.6 0.0068
-
 cephalothin beta-lactamase A Staphylococcus aureus
3.5.2.6 0.0079
-
 cephaloridine beta-lactamase D Staphylococcus aureus
3.5.2.6 0.011
-
 cephaloridine beta-lactamase A Staphylococcus aureus
3.5.2.6 0.0115
-
 cephaloridine beta-lactamase C Staphylococcus aureus
3.5.2.6 0.0122
-
 cephapirin beta-lactamase D Staphylococcus aureus
3.5.2.6 0.0157
-
 nitrocefin beta-lactamase B Staphylococcus aureus
3.5.2.6 0.018
-
 cefazolin beta-lactamase A Staphylococcus aureus
3.5.2.6 0.0194
-
 cephaloridine beta-lactamase B Staphylococcus aureus
3.5.2.6 0.0204
-
 nitrocefin beta-lactamase C Staphylococcus aureus
3.5.2.6 0.0386
-
 penicillin G beta-lactamase B Staphylococcus aureus
3.5.2.6 0.0472
-
 cephapirin beta-lactamase C Staphylococcus aureus
3.5.2.6 0.0499
-
 cefazolin beta-lactamase D Staphylococcus aureus
3.5.2.6 0.0511
-
 penicillin G beta-lactamase A Staphylococcus aureus
3.5.2.6 0.0559
-
 penicillin G beta-lactamase C Staphylococcus aureus
3.5.2.6 0.0795
-
 cephapirin beta-lactamase B Staphylococcus aureus
3.5.2.6 0.1126
-
 penicillin G beta-lactamase D Staphylococcus aureus
3.5.2.6 0.122
-
 ampicillin beta-lactamase C Staphylococcus aureus
3.5.2.6 0.129
-
 ampicillin beta-lactamase D Staphylococcus aureus
3.5.2.6 0.16
-
 ampicillin beta-lactamase B Staphylococcus aureus
3.5.2.6 0.254
-
 cefazolin beta-lactamase B Staphylococcus aureus
3.5.2.6 0.255
-
 ampicillin beta-lactamase A Staphylococcus aureus
3.5.2.6 0.267
-
 cefazolin beta-lactamase C Staphylococcus aureus
3.5.2.6 0.378
-
 cefuroxime beta-lactamase A Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.2.6 extracellular
-
 Staphylococcus aureus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 30000
-
 SDS-PAGE Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Staphylococcus aureus
-
-
-
3.5.2.6 Staphylococcus aureus
-
 strains PC11, 22260, V137, FAR8, FAR10
-
3.5.2.6 Staphylococcus aureus PC1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 4 enzymes: beta-lactamase A-D Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.6 80
-
 substrate penicillin G, beta-lactamase D Staphylococcus aureus
3.5.2.6 243
-
 substrate penicillin G, beta-lactamase A Staphylococcus aureus
3.5.2.6 252
-
 substrate penicillin G, beta-lactamase B Staphylococcus aureus
3.5.2.6 364
-
 substrate penicillin G, beta-lactamase C Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Staphylococcus aureus (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Staphylococcus aureus PC1 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefamandole + H2O
-
 Staphylococcus aureus (2R)-2-[(R)-carboxy{[(2R)-2-hydroxy-2-phenylacetyl]amino}methyl]-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefazolin + H2O
-
 Staphylococcus aureus (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Staphylococcus aureus (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Staphylococcus aureus PC1 (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Staphylococcus aureus (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Staphylococcus aureus PC1 (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephalothin + H2O
-
 Staphylococcus aureus (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalothin + H2O
-
 Staphylococcus aureus PC1 (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephapirin + H2O
-
 Staphylococcus aureus (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy{2-[(pyridin-4-yl)sulfanyl]acetamido}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephapirin + H2O
-
 Staphylococcus aureus PC1 (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy{2-[(pyridin-4-yl)sulfanyl]acetamido}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Staphylococcus aureus (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 penicillin G + H2O
-
 Staphylococcus aureus (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.00667
-
 cephalothin beta-lactamase D Staphylococcus aureus
3.5.2.6 0.00833
-
 cefuroxime beta-lactamase A Staphylococcus aureus
3.5.2.6 0.0167
-
 cephalothin beta-lactamase A Staphylococcus aureus
3.5.2.6 0.05
-
 cefamandole beta-lactamase A Staphylococcus aureus
3.5.2.6 0.05
-
 cefamandole beta-lactamase D Staphylococcus aureus
3.5.2.6 0.1
-
 cephalothin beta-lactamase B Staphylococcus aureus
3.5.2.6 0.1
-
 cephalothin beta-lactamase C Staphylococcus aureus
3.5.2.6 0.25
-
 cephapirin beta-lactamase D Staphylococcus aureus
3.5.2.6 0.3
-
 cefamandole beta-lactamase C Staphylococcus aureus
3.5.2.6 0.35
-
 cefamandole beta-lactamase B Staphylococcus aureus
3.5.2.6 0.367
-
 cephapirin beta-lactamase A Staphylococcus aureus
3.5.2.6 1
-
 cefazolin beta-lactamase A Staphylococcus aureus
3.5.2.6 1.18
-
 cephapirin beta-lactamase C Staphylococcus aureus
3.5.2.6 1.25
-
 cephaloridine beta-lactamase D Staphylococcus aureus
3.5.2.6 1.82
-
 cephapirin beta-lactamase B Staphylococcus aureus
3.5.2.6 1.92
-
 cefazolin beta-lactamase D Staphylococcus aureus
3.5.2.6 1.95
-
 cephaloridine beta-lactamase A Staphylococcus aureus
3.5.2.6 3.95
-
 cefazolin beta-lactamase C Staphylococcus aureus
3.5.2.6 4.67
-
 cefazolin beta-lactamase B Staphylococcus aureus
3.5.2.6 5.6
-
 cephaloridine beta-lactamase C Staphylococcus aureus
3.5.2.6 6.65
-
 cephaloridine beta-lactamase B Staphylococcus aureus
3.5.2.6 13.7
-
 nitrocefin beta-lactamase C Staphylococcus aureus
3.5.2.6 16.5
-
 nitrocefin beta-lactamase B Staphylococcus aureus
3.5.2.6 23.2
-
 nitrocefin beta-lactamase A Staphylococcus aureus
3.5.2.6 25.6
-
 nitrocefin beta-lactamase D Staphylococcus aureus
3.5.2.6 46
-
 penicillin G beta-lactamase D Staphylococcus aureus
3.5.2.6 133
-
 ampicillin beta-lactamase D Staphylococcus aureus
3.5.2.6 156
-
 penicillin G beta-lactamase B Staphylococcus aureus
3.5.2.6 171
-
 penicillin G beta-lactamase A Staphylococcus aureus
3.5.2.6 210
-
 penicillin G beta-lactamase C Staphylococcus aureus
3.5.2.6 308
-
 ampicillin beta-lactamase A Staphylococcus aureus
3.5.2.6 355
-
 ampicillin beta-lactamase C Staphylococcus aureus
3.5.2.6 412
-
 ampicillin beta-lactamase B Staphylococcus aureus