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Literature summary extracted from

  • Fujii-Kuriyama, Y.; Yamamoto, M.; Sugawara, S.
    Purification and properties of beta-lactamase from Proteus morganii (1977), J. Bacteriol., 131, 726-734.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 cefoxitin
-
 Morganella morganii
3.5.2.6 cephamycin C
-
 Morganella morganii
3.5.2.6 Cloxacillin
-
 Morganella morganii
3.5.2.6 Methicillin
-
 Morganella morganii
3.5.2.6 Oxacillin
-
 Morganella morganii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.0017
-
 cephalexin
-
 Morganella morganii
3.5.2.6 0.0059
-
 cephalothin
-
 Morganella morganii
3.5.2.6 0.028
-
 cefazolin
-
 Morganella morganii
3.5.2.6 0.084
-
 cephaloridine
-
 Morganella morganii
3.5.2.6 0.09
-
 ampicillin
-
 Morganella morganii
3.5.2.6 0.43
-
 benzylpenicillin
-
 Morganella morganii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 38000
-
 gel filtration Morganella morganii
3.5.2.6 40000
-
 SDS-PAGE Morganella morganii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.6 ampicillin + H2O Morganella morganii
-
 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O Morganella morganii 1510
-
 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O Morganella morganii
-
 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O Morganella morganii 1510
-
 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cephaloridine + H2O Morganella morganii
-
 (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephaloridine + H2O Morganella morganii 1510
-
 (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Morganella morganii
-
-
-
3.5.2.6 Morganella morganii 1510
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
 Morganella morganii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.6 190
-
-
 Morganella morganii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Morganella morganii (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Morganella morganii 1510 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Morganella morganii (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 benzylpenicillin + H2O
-
 Morganella morganii 1510 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefazolin + H2O
-
 Morganella morganii (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalexin + H2O
-
 Morganella morganii (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalexin + H2O
-
 Morganella morganii 1510 (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Morganella morganii (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Morganella morganii 1510 (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephalosporin C + H2O
-
 Morganella morganii N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine
-
 ?
3.5.2.6 cephalothin + H2O
-
 Morganella morganii (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalothin + H2O
-
 Morganella morganii 1510 (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephradine + H2O
-
 Morganella morganii (2R)-2-[(R)-{[(2R)-2-amino-2-(cyclohexa-1,4-dien-1-yl)acetyl]amino}(carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.6 monomer 1* 40000, SDS-PAGE Morganella morganii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.6 50
-
-
 Morganella morganii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.6 8.5
-
-
 Morganella morganii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.2.6 6 10
-
 Morganella morganii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.2.6 6.5 10 35% loss of activity at pH 6.4 and 10 Morganella morganii