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Literature summary extracted from

  • Seung-Goo, L.; Dong-Cheol, L.; Hak-Sung, K.
    Purification and characterization of thermostable D-hydantoinase from thermophilic Bacillus stearothermophilus SD-1 (1997), Appl. Biochem. Biotechnol., 62, 251-266.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.2.2 synthesis
-
 Thermus sp.
3.5.2.2 synthesis commercial production of optically pure amino acids for synthesis of antibiotics, pharmaceuticals, artificial sweeteners and biologically active peptides, food ingredients, pyrethroids, pesticides, antimicrobial and antiviral agents and other agrochemicals Geobacillus stearothermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.2
-
 Geobacillus stearothermophilus
3.5.2.2 D-hydantoinase genes cloned and sequenced, Escherichia coli transformed with cosmid vectors, D-hydantoinase expressed and identified Thermus sp.
3.5.2.2 expressed in Escherichia coli BL21 Thermus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.2 Cu2+
-
 Geobacillus stearothermophilus
3.5.2.2 Cu2+
-
 Pseudomonas fluorescens
3.5.2.2 N-Carbamoyl-glycine competitive inhibition Geobacillus stearothermophilus
3.5.2.2 Zn2+
-
 Geobacillus stearothermophilus
3.5.2.2 Zn2+
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.2 1.1
-
 dihydrouracil
-
 Geobacillus stearothermophilus
3.5.2.2 32.1
-
 phenylhydantoin
-
 Geobacillus stearothermophilus
3.5.2.2 47.2
-
 p-hydroxyphenylhydantoin
-
 Geobacillus stearothermophilus
3.5.2.2 340
-
 hydantoin
-
 Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.2 Mn2+
-
 Bos taurus
3.5.2.2 Mn2+
-
 Thermus sp.
3.5.2.2 Mn2+ requires 1 ion per molecule of enzyme for activity Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.2 51050
-
 determined by mass spectrometer Geobacillus stearothermophilus
3.5.2.2 51100
-
 2 * 51100, mass spectrometric analysis Geobacillus stearothermophilus
3.5.2.2 54000
-
 2 * 54000, SDS-PAGE Geobacillus stearothermophilus
3.5.2.2 102100
-
 mass spectrometric analysis Geobacillus stearothermophilus
3.5.2.2 133900
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O Bos taurus
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Geobacillus stearothermophilus
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Pseudomonas fluorescens
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Pseudomonas putida
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Thermus sp.
-
 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O Geobacillus stearothermophilus SD-1
-
 3-ureidopropionate
-
 ?
3.5.2.2 hydantoin + H2O Rattus norvegicus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Bos taurus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Geobacillus stearothermophilus
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas fluorescens
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Pseudomonas putida
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Thermus sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Agrobacterium sp.
-
 N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O Geobacillus stearothermophilus SD-1
-
 N-carbamoyl glycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Agrobacterium sp.
-
-
-
3.5.2.2 Bos taurus
-
 calf
-
3.5.2.2 Bos taurus
-
 bovine
-
3.5.2.2 Geobacillus stearothermophilus
-
-
-
3.5.2.2 Geobacillus stearothermophilus SD-1
-
-
-
3.5.2.2 Pseudomonas fluorescens
-
-
-
3.5.2.2 Pseudomonas putida
-
-
-
3.5.2.2 Rattus norvegicus
-
-
-
3.5.2.2 Thermus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2
-
 Geobacillus stearothermophilus
3.5.2.2
-
 Pseudomonas fluorescens
3.5.2.2
-
 Pseudomonas putida
3.5.2.2
-
 Agrobacterium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.2.2 liver
-
 Rattus norvegicus
-
3.5.2.2 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.2 148.6
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O
-
 Rattus norvegicus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Bos taurus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Geobacillus stearothermophilus 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas fluorescens 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Pseudomonas putida 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Thermus sp. 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Agrobacterium sp. 3-ureidopropionate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O
-
 Geobacillus stearothermophilus SD-1 3-ureidopropionate
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-(p-hydroxyphenyl)hydantoin + H2O
-
 Geobacillus stearothermophilus SD-1 N-carbamoyl-p-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Rattus norvegicus N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Bos taurus N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Pseudomonas putida N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-5-phenylhydantoin + H2O
-
 Geobacillus stearothermophilus SD-1 N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Bos taurus hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas fluorescens hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Thermus sp. hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus SD-1 hydantoic acid
-
 ?
3.5.2.2 hydantoin + H2O
-
 Rattus norvegicus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Bos taurus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas fluorescens N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Pseudomonas putida N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Thermus sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Agrobacterium sp. N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O
-
 Geobacillus stearothermophilus SD-1 N-carbamoyl glycine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.2 dimer
-
 Thermus sp.
3.5.2.2 dimer 2 * 54000, SDS-PAGE Geobacillus stearothermophilus
3.5.2.2 dimer 2 * 51100, mass spectrometric analysis Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.2 65
-
 hydantoin as substrate Geobacillus stearothermophilus
3.5.2.2 70
-
-
 Agrobacterium sp.
3.5.2.2 70
-
 DL-p-hydroxyphenylhydantoin as substrate Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.2 60
-
-
 Pseudomonas putida
3.5.2.2 60
-
 stable up to Geobacillus stearothermophilus
3.5.2.2 70
-
 treatment for 30 min, 20% activity remains Agrobacterium sp.
3.5.2.2 80
-
 half-life 30 min Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.2 8
-
-
 Geobacillus stearothermophilus
3.5.2.2 8
-
-
 Pseudomonas fluorescens
3.5.2.2 8.8
-
-
 Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.2.2 5.5
-
 remains stable at pH values higher than Geobacillus stearothermophilus