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Literature summary extracted from

  • Hirrlinger, B.; Stolz, A.; Knackmuss, H.J.
    Purification and properties of an amidase from Rhodococcus erythropolis MP50 which enantioselectively hydrolyzes 2-arylpropionamides (1996), J. Bacteriol., 178, 3501-3507.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.4 Ag+ 0% of origin activity Rhodococcus erythropolis
3.5.1.4 Cu2+ 0% of origin activity Rhodococcus erythropolis
3.5.1.4 dithiothreitol 97% of origin activity Rhodococcus erythropolis
3.5.1.4 iodoacetate 81% of origin activity Rhodococcus erythropolis
3.5.1.4 p-hydroxymercuribenzoate 53% of origin activity Rhodococcus erythropolis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 0.067
-
 ketoprofen amide
-
 Rhodococcus erythropolis
3.5.1.4 0.069
-
 phenylacetamide
-
 Rhodococcus erythropolis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 61000
-
 8 * 61000, SDS-PAGE Rhodococcus erythropolis
3.5.1.4 480000
-
 gel filtration Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.4 ketoprofen amide Rhodococcus erythropolis
-
 2-(3-benzoylphenyl)propionic acid + NH3
-
 ?
3.5.1.4 ketoprofen amide Rhodococcus erythropolis MP50
-
 2-(3-benzoylphenyl)propionic acid + NH3
-
 ?
3.5.1.4 phenylacetamide Rhodococcus erythropolis
-
 phenylacetic acid + NH3
-
 ?
3.5.1.4 phenylacetamide Rhodococcus erythropolis MP50
-
 phenylacetic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Rhodococcus erythropolis
-
-
-
3.5.1.4 Rhodococcus erythropolis MP50
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4 ion-exchange chromatography, phenyl-Superose column, desalted with a Hitrap desalting column Rhodococcus erythropolis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.4 0.18
-
 substrate urea Rhodococcus erythropolis
3.5.1.4 0.67
-
 activity in cell extract, substrate 1 mM phenylacetamide Rhodococcus erythropolis
3.5.1.4 0.81
-
 activity in cell extract, substrate 1 mM ketoprofen amide Rhodococcus erythropolis
3.5.1.4 1.3
-
 activity in cell extract, substrate 1 mM naproxen amide Rhodococcus erythropolis
3.5.1.4 4.1
-
 activity after purification, substrate 5 mM phenylacetamide Rhodococcus erythropolis
3.5.1.4 4.83
-
 substrate methacrylamide Rhodococcus erythropolis
3.5.1.4 6.69
-
 substrate cyclohexanamide Rhodococcus erythropolis
3.5.1.4 16.39
-
 substrate hexanamide Rhodococcus erythropolis

Storage Stability

EC Number Storage Stability Organism
3.5.1.4 room temperature, half-life about 2000 days with ammonium sulfate Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 ketoprofen amide
-
 Rhodococcus erythropolis 2-(3-benzoylphenyl)propionic acid + NH3
-
 ?
3.5.1.4 ketoprofen amide
-
 Rhodococcus erythropolis MP50 2-(3-benzoylphenyl)propionic acid + NH3
-
 ?
3.5.1.4 phenylacetamide
-
 Rhodococcus erythropolis phenylacetic acid + NH3
-
 ?
3.5.1.4 phenylacetamide
-
 Rhodococcus erythropolis MP50 phenylacetic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.4 octamer 8 * 61000, SDS-PAGE Rhodococcus erythropolis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.4 55
-
-
 Rhodococcus erythropolis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.4 55 65
-
 Rhodococcus erythropolis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.4 7.5
-
-
 Rhodococcus erythropolis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.4 2 10
-
 Rhodococcus erythropolis