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Literature summary extracted from

  • Kaul, R.; Casanova, J.; Johnson, A.B.; Tang, P.; Matalon, R.
    Purification, characterization, and localization of aspartoacylase from bovine brain (1991), J. Neurochem., 56, 129-135.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.15 dithiothreitol enhances activity Bos taurus
3.5.1.15 NP-40 enhances activity Bos taurus
3.5.1.15 Triton X-100 increases activity of the enzyme Bos taurus
3.5.1.15 Tween-20 enhances activity Bos taurus

Application

EC Number Application Comment Organism
3.5.1.15 medicine diagnosis of Canavan disease, an autosomal recessive neurodegenerative disorder characterized by aspartoacylase deficiency, technique for prenatal diagnosis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.15 Glycyl-L-aspartate
-
 Bos taurus
3.5.1.15 N-carbobenzoxy-D-aspartic acid
-
 Bos taurus
3.5.1.15 N-tert-butoxycarbonyl-D-aspartic acid-beta-benzyl ester
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.15 0.85
-
 N-acyl-L-aspartic acid
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.15 Ca2+ stimulates activity 20-40% Bos taurus
3.5.1.15 Mg2+ stimulates activity 20-40% Bos taurus
3.5.1.15 Mn2+ stimulates activity 20-40%, above 0.1 mM inhibition Bos taurus
3.5.1.15 Zn2+ stimulates activity 20-40%, above 0.1 mM inhibition Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.15 58000
-
 SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.15 N-acetyl-L-aspartic acid + H2O Homo sapiens
-
 acetate + aspartic acid
-
 ?
3.5.1.15 N-acetyl-L-aspartic acid + H2O Bos taurus
-
 acetate + aspartic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.15 Bos taurus
-
 bovine
-
3.5.1.15 Homo sapiens
-
 human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.15
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.15 brain
-
 Homo sapiens
-
3.5.1.15 brain
-
 Bos taurus
-
3.5.1.15 skin fibroblast
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.15 0.019 0.02
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.15 N-acetyl-L-aspartic acid + H2O
-
 Homo sapiens acetate + aspartic acid
-
 ?
3.5.1.15 N-acetyl-L-aspartic acid + H2O
-
 Bos taurus acetate + aspartic acid
-
 ?
3.5.1.15 N-acyl L-aspartic acid + H2O
-
 Homo sapiens acetate + L-aspartate
-
 ?
3.5.1.15 N-acyl L-aspartic acid + H2O
-
 Bos taurus acetate + L-aspartate
-
 ?
3.5.1.15 N-carbobenzoxy-L-aspartate + H2O
-
 Bos taurus ?
-
 ?
3.5.1.15 N-tert-butoxycarbonyl-L-aspartic acid alpha-benzyl ester + H2O
-
 Bos taurus ?
-
 ?
3.5.1.15 N-tert-butoxycarbonyl-L-aspartic acid beta-benzyl ester + H2O
-
 Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.15 monomer 1 * 58000, SDS-PAGE, reducing conditions Bos taurus