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Literature summary extracted from

  • Sakamoto, T.; Araki, C.; Beppu, T.; Arima, K.
    Partial purification and some properties of extracellular asparaginase from Candida utilis (1977), Agric. Biol. Chem., 41, 1359-1364.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 Hg2+ 1 mM, 80% loss of activity. 3 mM, complete inhibition Cyberlindnera jadinii
3.5.1.1 pyruvate 10 mM, 26% inhibition Cyberlindnera jadinii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.1 extracellular
-
 Cyberlindnera jadinii
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Cyberlindnera jadinii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 partial Cyberlindnera jadinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 D-Asn + H2O
-
 Cyberlindnera jadinii D-Asp + NH3
-
 ?
3.5.1.1 L-Asn + H2O
-
 Cyberlindnera jadinii L-Asp + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 50
-
 about Cyberlindnera jadinii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.1 37
-
 pH 4.0-10.0, 20 h, stable Cyberlindnera jadinii
3.5.1.1 50
-
 pH 4.0-10.0, 10 min, stable Cyberlindnera jadinii
3.5.1.1 55
-
 pH 7.0, stable Cyberlindnera jadinii
3.5.1.1 60
-
 pH 5.0-7.0, 10 min, stable Cyberlindnera jadinii
3.5.1.1 65
-
 pH 7.0, 10 min, 50% loss of activity Cyberlindnera jadinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 6
-
 hydrolysis of D-Asn and L-Asn Cyberlindnera jadinii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 4 8 pH 4.0: about 50% of maximal activity, pH 8.0: about 60% of maximal activity, hydrolysis of L-Asn. pH 4.0: about 40% of maximal activity, pH 8: about 50% of maximal activity, hydrolysis of D-Asn Cyberlindnera jadinii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.1 4 10 50°C, 10 min, stable, 37°C, 20 h, stable Cyberlindnera jadinii
3.5.1.1 5 7 60°C, 10 min, stable Cyberlindnera jadinii