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Literature summary extracted from

  • Blee, E.; Schuber, F.
    Occurence of fatty acid epoxide hydrolases in soybean (Glycine max) (1992), Biochem. J., 282, 711-714.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.3.2.10 m-chloroperbenzoic acid
-
 Glycine max
3.3.2.10 styrene oxide inhibits hydration of trans-9,10-epoxystearate Glycine max
3.3.2.10 trans-9,10-epoxystearate 1 mM, 20% inhibition of hydration of 0.025 mM cis-9,10-epoxystearate Glycine max
3.3.2.10 trans-stilbene oxide inhibits hydration of trans-9,10-epoxystearate Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.10 0.026
-
 cis-9,10-epoxystearate microsomal enzyme Glycine max
3.3.2.10 0.045
-
 cis-9,10-epoxystearate soluble enzyme Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.3.2.10 cytosol
-
 Glycine max 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.3.2.10 32000
-
 2 * 32000, SDS-PAGE Glycine max
3.3.2.10 64200
-
 gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.10 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.10
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.3.2.10 seedling
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.10 cis-9,10-epoxystearate + H2O
-
 Glycine max threo-9,10-dihydroxystearate
-
 ?
3.3.2.10 trans-9,10-epoxystearate + H2O
-
 Glycine max threo-9,10-dihydroxystearate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.3.2.10 dimer 2 * 32000, SDS-PAGE Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.10 7.5
-
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.3.2.10 5.5 9.5 pH 5.5: about 30% of maximal activity, pH 9.5: about 25% of maximal activity Glycine max