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Literature summary extracted from

  • Cousineau, J.; Green, J.R.
    Isolation and characterization of the proximal and distal forms of lactase-phlorizin hydrolase from the small intestine of the suckling rat (1980), Biochim. Biophys. Acta, 615, 147-157.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.62 0.73
-
 N-palmitoyldihydrocerebroside from proximal intestine isolated phlorizin hydrolase activity Rattus sp.
3.2.1.62 1.12
-
 N-palmitoyldihydrocerebroside from distal intestine isolated phlorizin hydrolase activity Rattus sp.
3.2.1.62 14.8
-
 4-O-beta-D-galactopyranosyl-D-glucopyranoside from proximal intestine isolated lactase Rattus sp.
3.2.1.62 19.1
-
 4-O-beta-D-galactopyranosyl-D-glucopyranoside from distal intestine isolated lactase Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.62 brush border
-
 Rattus sp. 5903
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Rattus sp.
-
 D-galactose + beta-D-glucose
-
 ?
3.2.1.62 N-palmitoyldihydroglucosylceramide + H2O Rattus sp.
-
 beta-D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.62 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.62 two isoenzymes from proximal and distal intestine, to homogeneity, chromatography techniques Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.62 intestine
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.62 22
-
-
 Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O
-
 Rattus sp. D-galactose + beta-D-glucose
-
 ?
3.2.1.62 N-palmitoyldihydroglucosylceramide + H2O
-
 Rattus sp. beta-D-glucose + ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.62 45
-
 stable for 60 min Rattus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.62 6
-
 both isoforms Rattus sp.