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Literature summary extracted from

  • Day, A.J.; Canada, F.J.; Diaz, J.C.; Kroon, P.A.; Mclauchlan, R.; Faulds, C.B.; Plumb, G.W.; Morgan, M.R.A.; Williamson, G.
    Dietary flavonoids and isoflavone glycosides are hydrolysed by the lactase site of lactase phlorizin hydrolase (2000), FEBS Lett., 468, 166-170.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.62 2',4'-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside rapidly inhibits phlorizin hydrolase activity by covalently binding to the glutamic acid residue of the active site, lactase activity more slowly inhibited, pre-incubation with glucal protects the lactase active site from inactivation Ovis aries aries
3.2.1.62 2-fluoro-dinitrophenylglucoside specifically inhibits phlorizin hydrolase, lactase activity is inhibited slowly Ovis aries
3.2.1.108 2-fluoro-dinitrophenylglucoside specifically inhibits phlorizin hydrolase, lactase activity is inhibited slowly Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.62 0.002
-
 phlorizin
-
 Ovis aries aries
3.2.1.62 0.002
-
 phlorizin pH 6, 37°C Ovis aries
3.2.1.62 0.044
-
 quercetin-4-glucopyranoside
-
 Ovis aries aries
3.2.1.62 0.044
-
 quercetin-4'-glucoside pH 6, 37°C Ovis aries
3.2.1.62 0.046
-
 quercetin 3-O-beta-D-glucopyranoside
-
 Ovis aries aries
3.2.1.62 0.046
-
 quercetin-3-glucoside pH 6, 37°C Ovis aries
3.2.1.62 0.085
-
 genistein-7-glucopyranoside
-
 Ovis aries aries
3.2.1.62 0.085
-
 genistein-7-glucoside pH 6, 37°C Ovis aries
3.2.1.62 0.149
-
 daidzein-7-glucopyranoside
-
 Ovis aries aries
3.2.1.62 0.149
-
 daidzein-7-glucoside pH 6, 37°C Ovis aries
3.2.1.62 8.8
-
 4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
 Ovis aries aries
3.2.1.62 8.8
-
 lactose pH 6, 37°C Ovis aries
3.2.1.108 0.002
-
 phlorizin pH 6, 37°C Ovis aries
3.2.1.108 0.044
-
 quercetin-4'-glucoside pH 6, 37°C Ovis aries
3.2.1.108 0.046
-
 quercetin-3-glucoside pH 6, 37°C Ovis aries
3.2.1.108 0.085
-
 genistein-7-glucoside pH 6, 37°C Ovis aries
3.2.1.108 0.149
-
 daidzein-7-glucoside pH 6, 37°C Ovis aries
3.2.1.108 8.8
-
 lactose pH 6, 37°C Ovis aries

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.62 brush border luminal site Ovis aries aries 5903
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Ovis aries aries hydrolysis located on a specific active site for lactose hydrolysis D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O Ovis aries aries acts on dietary glycosides before absorption D-galactose + beta-D-glucose
-
 ?
3.2.1.62 phlorizin + H2O Ovis aries aries hydrolysis located on a specific active site for phlorizin hydrolysis phloretin + beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.62 Ovis aries
-
-
-
3.2.1.62 Ovis aries aries
-
 sheep
-
3.2.1.108 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.62 to homogeneity Ovis aries aries

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.62 a beta-D-glucosyl-N-acylsphingosine + H2O = a ceramide + beta-D-glucose mechanism Ovis aries
a
3.2.1.108 lactose + H2O = D-galactose + D-glucose mechanism Ovis aries
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.62 intestine small intestine Ovis aries aries
-
3.2.1.62 small intestine
-
 Ovis aries
-
3.2.1.108 small intestine
-
 Ovis aries
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O hydrolysis located on a specific active site for lactose hydrolysis Ovis aries aries D-galactose + beta-D-glucose
-
 ?
3.2.1.62 4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O acts on dietary glycosides before absorption Ovis aries aries D-galactose + beta-D-glucose
-
 ?
3.2.1.62 daidzein 7-O-beta-D-glucopyranoside + H2O
-
 Ovis aries aries daidzein + beta-D-glucose
-
 ?
3.2.1.62 daidzin + H2O
-
 Ovis aries 3-(4-hydroxyphenyl)-7-hydroxy-chromen-4-one + D-glucose
-
 ?
3.2.1.62 genistein-7-glucopyranoside + H2O
-
 Ovis aries aries genistein + beta-D-glucose
-
 ?
3.2.1.62 genistein-7-glucoside + H2O
-
 Ovis aries genistein + D-glucose
-
 ?
3.2.1.62 lactose + H2O reaction is not inhibited by 2-fluoro-dinitrophenylglucoside Ovis aries D-galactose + glucose
-
 ?
3.2.1.62 additional information no substrate: rutin, naringin Ovis aries ?
-
 ?
3.2.1.62 phlorizin + H2O hydrolysis located on a specific active site for phlorizin hydrolysis Ovis aries aries phloretin + beta-D-glucose
-
 ?
3.2.1.62 phlorizin + H2O reaction is inhibited by 2-fluoro-dinitrophenylglucoside Ovis aries ?
-
 ?
3.2.1.62 quercetin 3-O-beta-D-glucopyranoside + H2O
-
 Ovis aries aries quercetin + beta-D-glucose
-
 ?
3.2.1.62 quercetin 4'-O-beta-D-glucopyranoside + H2O flavone glucosides more efficiently hydrolysed than isoflavone glucosides, major contribution to the hydrolysis is from the lactase site Ovis aries aries quercetin + beta-D-glucose
-
 ?
3.2.1.62 quercetin-3-glucoside + H2O reaction is marginally inhibited by 2-fluoro-dinitrophenylglucoside Ovis aries quercetin + D-glucose
-
 ?
3.2.1.62 quercetin-4'-glucoside + H2O reaction is marginally inhibited by 2-fluoro-dinitrophenylglucoside Ovis aries quercetin + D-glucose
-
 ?
3.2.1.108 daidzein 7-O-beta-D-glucopyranoside + H2O
-
 Ovis aries daidzein + beta-D-glucose
-
 ?
3.2.1.108 genistein-7-glucoside + H2O
-
 Ovis aries genistein + D-glucose
-
 ?
3.2.1.108 lactose + H2O reaction is not inhibited by 2-fluoro-dinotrophenylglucoside Ovis aries galactose + D-glucose
-
 ?
3.2.1.108 additional information no substrate: rutin, naringin Ovis aries ?
-
 ?
3.2.1.108 phlorizin + H2O reaction is inhibited by 2-fluoro-dinotrophenylglucoside Ovis aries ?
-
 ?
3.2.1.108 quercetin-3-glucoside + H2O reaction is marginally inhibited by 2-fluoro-dinotrophenylglucoside Ovis aries quercetin + D-glucose
-
 ?
3.2.1.108 quercetin-4'-glucoside + H2O reaction is marginally inhibited by 2-fluoro-dinotrophenylglucoside Ovis aries quercetin + D-glucose
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.62 0.5
-
 phlorizin
-
 Ovis aries aries
3.2.1.62 0.5
-
 phlorizin pH 6, 37°C Ovis aries
3.2.1.62 2.1
-
 daidzein-7-beta-D-glucopyranoside
-
 Ovis aries aries
3.2.1.62 2.1
-
 daidzein-7-glucoside pH 6, 37°C Ovis aries
3.2.1.62 6.3
-
 quercetin 3-O-beta-D-glucopyranoside
-
 Ovis aries aries
3.2.1.62 6.3
-
 quercetin-3-glucoside pH 6, 37°C Ovis aries
3.2.1.62 6.5
-
 genistein-7-beta-D-glucopyranoside
-
 Ovis aries aries
3.2.1.62 6.5
-
 genistein-7-glucoside pH 6, 37°C Ovis aries
3.2.1.62 7.5
-
 quercetin-4-beta-D-glucopyranoside
-
 Ovis aries aries
3.2.1.62 7.5
-
 quercetin-4'-glucoside pH 6, 37°C Ovis aries
3.2.1.62 33.8
-
 4-O-beta-D-galactopyranosyl-D-glucopyranoside
-
 Ovis aries aries
3.2.1.62 33.8
-
 lactose pH 6, 37°C Ovis aries
3.2.1.108 0.5
-
 phlorizin pH 6, 37°C Ovis aries
3.2.1.108 2.1
-
 daidzein-7-glucoside pH 6, 37°C Ovis aries
3.2.1.108 6.3
-
 quercetin-3-glucoside pH 6, 37°C Ovis aries
3.2.1.108 6.5
-
 genistein-7-glucoside pH 6, 37°C Ovis aries
3.2.1.108 7.5
-
 quercetin-4'-glucoside pH 6, 37°C Ovis aries
3.2.1.108 33.8
-
 lactose pH 6, 37°C Ovis aries