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Literature summary extracted from

  • Yoshima, H.; Takasaki, S.; Ito-Mega, S.; Kobata, A.
    Purification of almond emulsin alpha-L-fucosidase I by affinity chromatography (1979), Arch. Biochem. Biophys., 194, 394-398.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O Prunus dulcis alpha-fucosidase I alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O Prunus dulcis lacto-N-fucopentaose I alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 lacto-N-fucopentaose II + H2O Prunus dulcis alpha-fucosidase II alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.111 additional information Prunus dulcis the enzyme hydrolyzes fucose alpha-1,3 or alpha-1,4-linkages to N-acteylglucosamine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Prunus dulcis
-
 almond
-
3.2.1.111 Prunus dulcis
-
 almond
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51 to homogeneity, affinity chromatography, alpha-fucosidase I Prunus dulcis
3.2.1.111 partial Prunus dulcis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 emulsin two forms, alpha-fucosidase I and II Prunus dulcis
-
3.2.1.111 emulsin
-
 Prunus dulcis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.111 additional information
-
-
 Prunus dulcis

Storage Stability

EC Number Storage Stability Organism
3.2.1.111 frozen, retains more than 90% of activity after 1 month Prunus dulcis
3.2.1.111 storage of lyophilized enzyme preparation results in 60% loss of activity within 1 month Prunus dulcis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O alpha-fucosidase I Prunus dulcis alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaose I Prunus dulcis alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.51 lacto-N-fucopentaose II + H2O alpha-fucosidase II Prunus dulcis alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
3.2.1.111 lacto-N-fucopentaitol + H2O
-
 Prunus dulcis ?
-
 ?
3.2.1.111 additional information the enzyme hydrolyzes fucose alpha-1,3 or alpha-1,4-linkages to N-acteylglucosamine Prunus dulcis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.111 37
-
 assay at Prunus dulcis