BRENDA - Enzyme Database

Purification of almond emulsin alpha-L-fucosidase I by affinity chromatography

Yoshima, H.; Takasaki, S.; Ito-Mega, S.; Kobata, A.; Arch. Biochem. Biophys. 194, 394-398 (1979)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Prunus dulcis
alpha-fucosidase I
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Prunus dulcis
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Prunus dulcis
lacto-N-fucopentaose I
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Prunus dulcis
?
3.2.1.51
lacto-N-fucopentaose II + H2O
Prunus dulcis
alpha-fucosidase II
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Prunus dulcis
?
3.2.1.111
additional information
Prunus dulcis
the enzyme hydrolyzes fucose alpha-1,3 or alpha-1,4-linkages to N-acteylglucosamine
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Prunus dulcis
-
almond
-
3.2.1.111
Prunus dulcis
-
almond
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography, alpha-fucosidase I
Prunus dulcis
3.2.1.111
partial
Prunus dulcis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
emulsin
two forms, alpha-fucosidase I and II
Prunus dulcis
-
3.2.1.111
emulsin
-
Prunus dulcis
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.111
additional information
-
-
Prunus dulcis
Storage Stability
EC Number
Storage Stability
Organism
3.2.1.111
frozen, retains more than 90% of activity after 1 month
Prunus dulcis
3.2.1.111
storage of lyophilized enzyme preparation results in 60% loss of activity within 1 month
Prunus dulcis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-fucosidase I
208728
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208728
Prunus dulcis
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose I
208728
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208728
Prunus dulcis
?
3.2.1.51
lacto-N-fucopentaose II + H2O
alpha-fucosidase II
208728
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208728
Prunus dulcis
?
3.2.1.111
lacto-N-fucopentaitol + H2O
-
208728
Prunus dulcis
?
-
-
-
?
3.2.1.111
additional information
the enzyme hydrolyzes fucose alpha-1,3 or alpha-1,4-linkages to N-acteylglucosamine
208728
Prunus dulcis
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.111
37
-
assay at
Prunus dulcis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Prunus dulcis
alpha-fucosidase I
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Prunus dulcis
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Prunus dulcis
lacto-N-fucopentaose I
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Prunus dulcis
?
3.2.1.51
lacto-N-fucopentaose II + H2O
Prunus dulcis
alpha-fucosidase II
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Prunus dulcis
?
3.2.1.111
additional information
Prunus dulcis
the enzyme hydrolyzes fucose alpha-1,3 or alpha-1,4-linkages to N-acteylglucosamine
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography, alpha-fucosidase I
Prunus dulcis
3.2.1.111
partial
Prunus dulcis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
emulsin
two forms, alpha-fucosidase I and II
Prunus dulcis
-
3.2.1.111
emulsin
-
Prunus dulcis
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.111
additional information
-
-
Prunus dulcis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.1.111
frozen, retains more than 90% of activity after 1 month
Prunus dulcis
3.2.1.111
storage of lyophilized enzyme preparation results in 60% loss of activity within 1 month
Prunus dulcis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-fucosidase I
208728
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208728
Prunus dulcis
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaose I
208728
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208728
Prunus dulcis
?
3.2.1.51
lacto-N-fucopentaose II + H2O
alpha-fucosidase II
208728
Prunus dulcis
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208728
Prunus dulcis
?
3.2.1.111
lacto-N-fucopentaitol + H2O
-
208728
Prunus dulcis
?
-
-
-
?
3.2.1.111
additional information
the enzyme hydrolyzes fucose alpha-1,3 or alpha-1,4-linkages to N-acteylglucosamine
208728
Prunus dulcis
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.111
37
-
assay at
Prunus dulcis