BRENDA - Enzyme Database

Substrate specificity and other properties of alpha-L-fucosidase from human serum

DiCioccio, R.A.; Barlow, J.J.; Matta, K.L.; J. Biol. Chem. 257, 714-718 (1982)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.18
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Homo sapiens
3.2.1.51
0.52
-
Fuc-alpha-(1-2)-Gal-beta-4-nitrophenol
-
Homo sapiens
3.2.1.51
1.56
-
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc
-
Homo sapiens
3.2.1.51
1.63
-
Fuc-alpha-(1-3)-GlcNAc-phenol
-
Homo sapiens
3.2.1.51
2.01
-
Fuc-alpha-(1-4)-GlcNAc-beta-phenol
-
Homo sapiens
3.2.1.51
2.9
-
Fuc-alpha-(1-2)-Gal
-
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
270000
-
gel filtration at pH 7.0
Homo sapiens
3.2.1.51
390000
-
gel filtration at pH 5.0
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal + H2O
Homo sapiens
-
alpha-L-fucose + D-galactose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
Homo sapiens
2'-fucosyllactose
alpha-L-fucose + lactose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Glc + H2O
Homo sapiens
-
alpha-L-fucose + D-glucose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Xyl + H2O
Homo sapiens
-
alpha-L-fucose + D-xylose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-Gal + H2O
Homo sapiens
-
alpha-L-fucose + D-galactose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-Glc + H2O
Homo sapiens
-
alpha-L-fucose + D-glucose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc + H2O
Homo sapiens
-
alpha-L-fucose + N-acetyl-D-glucosamine
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-4)-GlcNAc + H2O
Homo sapiens
-
alpha-L-fucose + N-acetyl-D-glucosamine
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-Gal + H2O
Homo sapiens
-
alpha-L-fucose + D-galactose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc + H2O
Homo sapiens
-
alpha-L-fucose + N-acetyl-D-glucosamine
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc-beta-Asn + H2O
Homo sapiens
-
alpha-L-fucose + N-acetyl-D-glucosaminyl-Asn
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
serum
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
10.66
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208719
Homo sapiens
4-nitrophenol + alpha-L-fucose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Fuc-beta-4-nitrophenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + Fuc-beta-4-nitrophenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal + H2O
-
208719
Homo sapiens
alpha-L-fucose + D-galactose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GalNAc-alpha-phenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + Gal-beta-(1-3)-N-acetyl-galactosaminyl-phenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
2'-fucosyllactose
208719
Homo sapiens
alpha-L-fucose + lactose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-4-nitrophenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + beta-galactosyl-4-nitrophenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Glc + H2O
-
208719
Homo sapiens
alpha-L-fucose + D-glucose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Xyl + H2O
-
208719
Homo sapiens
alpha-L-fucose + D-xylose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-[Gal-alpha-(1-3)]-Gal-beta-phenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + Gal-alpha-(1-3)-Gal-beta-phenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-Gal + H2O
-
208719
Homo sapiens
alpha-L-fucose + D-galactose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-Glc + H2O
-
208719
Homo sapiens
alpha-L-fucose + D-glucose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc + H2O
-
208719
Homo sapiens
alpha-L-fucose + N-acetyl-D-glucosamine
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc-beta-O(CH2)6NHCOCF3 + H2O
-
208719
Homo sapiens
alpha-L-fucose + GlcNAc-beta-O(CH2)6NHCOCF3
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc-beta-phenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + N-acetyl-glucosaminyl-beta-phenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-4)-GlcNAc + H2O
-
208719
Homo sapiens
alpha-L-fucose + N-acetyl-D-glucosamine
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-4)-GlcNAc-beta-phenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + beta-N-acetyl-glucosaminyl-phenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-4)-[Gal-beta-(1-3)]-GlcNAc-beta-phenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-phenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-Gal + H2O
-
208719
Homo sapiens
alpha-L-fucose + D-galactose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc + H2O
-
208719
Homo sapiens
alpha-L-fucose + N-acetyl-D-glucosamine
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc-4-nitrophenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + GlcNAc-4-nitrophenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc-beta-Asn + H2O
-
208719
Homo sapiens
alpha-L-fucose + N-acetyl-D-glucosaminyl-Asn
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-[Gal-beta-(1-3)]-GlcNAc-beta-OCH3 + H2O
-
208719
Homo sapiens
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-OCH3
-
208719
Homo sapiens
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
oligomer
contains subunits of 63 kDa, SDS-PAGE
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5
-
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.18
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Homo sapiens
3.2.1.51
0.52
-
Fuc-alpha-(1-2)-Gal-beta-4-nitrophenol
-
Homo sapiens
3.2.1.51
1.56
-
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc
-
Homo sapiens
3.2.1.51
1.63
-
Fuc-alpha-(1-3)-GlcNAc-phenol
-
Homo sapiens
3.2.1.51
2.01
-
Fuc-alpha-(1-4)-GlcNAc-beta-phenol
-
Homo sapiens
3.2.1.51
2.9
-
Fuc-alpha-(1-2)-Gal
-
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
270000
-
gel filtration at pH 7.0
Homo sapiens
3.2.1.51
390000
-
gel filtration at pH 5.0
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
Fuc-alpha-(1-2)-Gal + H2O
Homo sapiens
-
alpha-L-fucose + D-galactose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
Homo sapiens
2'-fucosyllactose
alpha-L-fucose + lactose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Glc + H2O
Homo sapiens
-
alpha-L-fucose + D-glucose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Xyl + H2O
Homo sapiens
-
alpha-L-fucose + D-xylose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-Gal + H2O
Homo sapiens
-
alpha-L-fucose + D-galactose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-Glc + H2O
Homo sapiens
-
alpha-L-fucose + D-glucose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc + H2O
Homo sapiens
-
alpha-L-fucose + N-acetyl-D-glucosamine
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-4)-GlcNAc + H2O
Homo sapiens
-
alpha-L-fucose + N-acetyl-D-glucosamine
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-Gal + H2O
Homo sapiens
-
alpha-L-fucose + D-galactose
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc + H2O
Homo sapiens
-
alpha-L-fucose + N-acetyl-D-glucosamine
-
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc-beta-Asn + H2O
Homo sapiens
-
alpha-L-fucose + N-acetyl-D-glucosaminyl-Asn
-
Homo sapiens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, affinity chromatography
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
serum
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
10.66
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208719
Homo sapiens
4-nitrophenol + alpha-L-fucose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Fuc-beta-4-nitrophenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + Fuc-beta-4-nitrophenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal + H2O
-
208719
Homo sapiens
alpha-L-fucose + D-galactose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GalNAc-alpha-phenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + Gal-beta-(1-3)-N-acetyl-galactosaminyl-phenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
2'-fucosyllactose
208719
Homo sapiens
alpha-L-fucose + lactose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-4-nitrophenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + beta-galactosyl-4-nitrophenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Glc + H2O
-
208719
Homo sapiens
alpha-L-fucose + D-glucose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-Xyl + H2O
-
208719
Homo sapiens
alpha-L-fucose + D-xylose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-2)-[Gal-alpha-(1-3)]-Gal-beta-phenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + Gal-alpha-(1-3)-Gal-beta-phenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-Gal + H2O
-
208719
Homo sapiens
alpha-L-fucose + D-galactose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-Glc + H2O
-
208719
Homo sapiens
alpha-L-fucose + D-glucose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc + H2O
-
208719
Homo sapiens
alpha-L-fucose + N-acetyl-D-glucosamine
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc-beta-O(CH2)6NHCOCF3 + H2O
-
208719
Homo sapiens
alpha-L-fucose + GlcNAc-beta-O(CH2)6NHCOCF3
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-3)-GlcNAc-beta-phenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + N-acetyl-glucosaminyl-beta-phenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-4)-GlcNAc + H2O
-
208719
Homo sapiens
alpha-L-fucose + N-acetyl-D-glucosamine
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-4)-GlcNAc-beta-phenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + beta-N-acetyl-glucosaminyl-phenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-4)-[Gal-beta-(1-3)]-GlcNAc-beta-phenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-phenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-Gal + H2O
-
208719
Homo sapiens
alpha-L-fucose + D-galactose
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc + H2O
-
208719
Homo sapiens
alpha-L-fucose + N-acetyl-D-glucosamine
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc-4-nitrophenol + H2O
-
208719
Homo sapiens
alpha-L-fucose + GlcNAc-4-nitrophenol
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-GlcNAc-beta-Asn + H2O
-
208719
Homo sapiens
alpha-L-fucose + N-acetyl-D-glucosaminyl-Asn
-
208719
Homo sapiens
?
3.2.1.51
Fuc-alpha-(1-6)-[Gal-beta-(1-3)]-GlcNAc-beta-OCH3 + H2O
-
208719
Homo sapiens
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-OCH3
-
208719
Homo sapiens
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
oligomer
contains subunits of 63 kDa, SDS-PAGE
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
5
-
-
Homo sapiens