BRENDA - Enzyme Database

Purification and characterization of a novel alpha-L-fucosidase from Fusarium oxysporum grown on sludge

Yano, T.; Yamamoto, K.; Kumagai, H.; Tochikura, T.; Yokoyama, T.; Seno, T.; Yamaguchi, H.; Agric. Biol. Chem. 49, 3179-3187 (1985)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
alpha-L-fucose
85% inhibition at 2.5 mM
Fusarium oxysporum
3.2.1.51
D-arabinose
95% inhibition at 2.5 mM
Fusarium oxysporum
3.2.1.51
Hg2+
marked inhibition at 20 mM
Fusarium oxysporum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.87
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Fusarium oxysporum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
extracellular
most induction of enzyme synthesis if cells grown in 2% active sludge hydrolyzate medium
Fusarium oxysporum
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
80000
-
gel filtration
Fusarium oxysporum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
saliva + H2O
Fusarium oxysporum
Ulex europaeus
alpha-L-fucose + ?
-
Fusarium oxysporum
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Fusarium oxysporum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
homogeneous, chromatography steps
Fusarium oxysporum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
cell culture
-
Fusarium oxysporum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.2.1.51
2.58
-
-
Fusarium oxysporum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208714
Fusarium oxysporum
4-nitrophenol + alpha-L-fucose
-
208714
Fusarium oxysporum
?
3.2.1.51
saliva + H2O
Ulex europaeus
208714
Fusarium oxysporum
alpha-L-fucose + ?
-
208714
Fusarium oxysporum
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.2.1.51
50
-
-
Fusarium oxysporum
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.2.1.51
45
-
stable up to
Fusarium oxysporum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
4.5
5.5
-
Fusarium oxysporum
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
4
8
stable within
Fusarium oxysporum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
alpha-L-fucose
85% inhibition at 2.5 mM
Fusarium oxysporum
3.2.1.51
D-arabinose
95% inhibition at 2.5 mM
Fusarium oxysporum
3.2.1.51
Hg2+
marked inhibition at 20 mM
Fusarium oxysporum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.87
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Fusarium oxysporum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
extracellular
most induction of enzyme synthesis if cells grown in 2% active sludge hydrolyzate medium
Fusarium oxysporum
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
80000
-
gel filtration
Fusarium oxysporum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
saliva + H2O
Fusarium oxysporum
Ulex europaeus
alpha-L-fucose + ?
-
Fusarium oxysporum
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
homogeneous, chromatography steps
Fusarium oxysporum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
cell culture
-
Fusarium oxysporum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.2.1.51
2.58
-
-
Fusarium oxysporum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208714
Fusarium oxysporum
4-nitrophenol + alpha-L-fucose
-
208714
Fusarium oxysporum
?
3.2.1.51
saliva + H2O
Ulex europaeus
208714
Fusarium oxysporum
alpha-L-fucose + ?
-
208714
Fusarium oxysporum
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.2.1.51
50
-
-
Fusarium oxysporum
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.2.1.51
45
-
stable up to
Fusarium oxysporum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
4.5
5.5
-
Fusarium oxysporum
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
4
8
stable within
Fusarium oxysporum