BRENDA - Enzyme Database

Purification and characterization of an alpha-L-fucosidase from Pomacea canaliculata

Endo, T.; Tsukada, T.; Hiraiwa, M.; Uda, Y.; Kobata, A.; Arch. Biochem. Biophys. 302, 152-160 (1993)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.2.1.51
analysis
studies of structures and functions of glycoconjugates and oligosaccharides due to unique substrate specificity
Pomacea canaliculata
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.45
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Pomacea canaliculata
3.2.1.51
1.18
-
2'-fucosyllactitol
-
Pomacea canaliculata
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
60000
-
4 * 60000, SDS-PAGE
Pomacea canaliculata
3.2.1.51
260000
-
gel filtration
Pomacea canaliculata
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
2'-fucosyllactitol + H2O
Pomacea canaliculata
-
alpha-L-fucose + lactitol
-
Pomacea canaliculata
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Pomacea canaliculata
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
Pomacea canaliculata
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Pomacea canaliculata
lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide
Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
Pomacea canaliculata
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Pomacea canaliculata
hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
Pomacea canaliculata
?
3.2.1.51
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
Pomacea canaliculata
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Pomacea canaliculata
?
3.2.1.51
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
Pomacea canaliculata
very slow hydrolysis, NaBH4-reduced oligosaccharide
Gal-beta-(1-4)-Glc + alpha-L-fucose
-
Pomacea canaliculata
?
3.2.1.51
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O
Pomacea canaliculata
-
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
-
Pomacea canaliculata
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Pomacea canaliculata
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, chromatography steps
Pomacea canaliculata
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
hepatopancreas
-
Pomacea canaliculata
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
5.3
-
-
Pomacea canaliculata
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
2'-fucosyllactitol + H2O
-
208702
Pomacea canaliculata
alpha-L-fucose + lactitol
-
208702
Pomacea canaliculata
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208702
Pomacea canaliculata
4-nitrophenol + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
208702
Pomacea canaliculata
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide
208702
Pomacea canaliculata
Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
NaBH4-reduced oligosaccharide, hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
208702
Pomacea canaliculata
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
208702
Pomacea canaliculata
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
208702
Pomacea canaliculata
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208702
Pomacea canaliculata
?
3.2.1.51
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
208702
Pomacea canaliculata
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208702
Pomacea canaliculata
?
3.2.1.51
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
NaBH4-reduced oligosaccharide, very slow hydrolysis
208702
Pomacea canaliculata
Gal-beta-(1-4)-Glc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
very slow hydrolysis, NaBH4-reduced oligosaccharide
208702
Pomacea canaliculata
Gal-beta-(1-4)-Glc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O
-
208702
Pomacea canaliculata
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O
subscript OT is used to indicate NaBH4-reduced oligosaccharide
208702
Pomacea canaliculata
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
tetramer
4 * 60000, SDS-PAGE
Pomacea canaliculata
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
2.5
-
two pH-optima
Pomacea canaliculata
3.2.1.51
5
-
two pH-optima
Pomacea canaliculata
Application (protein specific)
EC Number
Application
Commentary
Organism
3.2.1.51
analysis
studies of structures and functions of glycoconjugates and oligosaccharides due to unique substrate specificity
Pomacea canaliculata
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.45
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Pomacea canaliculata
3.2.1.51
1.18
-
2'-fucosyllactitol
-
Pomacea canaliculata
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
60000
-
4 * 60000, SDS-PAGE
Pomacea canaliculata
3.2.1.51
260000
-
gel filtration
Pomacea canaliculata
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.51
2'-fucosyllactitol + H2O
Pomacea canaliculata
-
alpha-L-fucose + lactitol
-
Pomacea canaliculata
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Pomacea canaliculata
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
Pomacea canaliculata
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Pomacea canaliculata
lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide
Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
Pomacea canaliculata
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Pomacea canaliculata
hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
Pomacea canaliculata
?
3.2.1.51
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
Pomacea canaliculata
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
Pomacea canaliculata
?
3.2.1.51
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
Pomacea canaliculata
very slow hydrolysis, NaBH4-reduced oligosaccharide
Gal-beta-(1-4)-Glc + alpha-L-fucose
-
Pomacea canaliculata
?
3.2.1.51
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O
Pomacea canaliculata
-
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
-
Pomacea canaliculata
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
to homogeneity, chromatography steps
Pomacea canaliculata
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.51
hepatopancreas
-
Pomacea canaliculata
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.51
5.3
-
-
Pomacea canaliculata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
2'-fucosyllactitol + H2O
-
208702
Pomacea canaliculata
alpha-L-fucose + lactitol
-
208702
Pomacea canaliculata
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
208702
Pomacea canaliculata
4-nitrophenol + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
208702
Pomacea canaliculata
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide
208702
Pomacea canaliculata
Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
NaBH4-reduced oligosaccharide, hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
208702
Pomacea canaliculata
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
208702
Pomacea canaliculata
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
208702
Pomacea canaliculata
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208702
Pomacea canaliculata
?
3.2.1.51
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
208702
Pomacea canaliculata
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
208702
Pomacea canaliculata
?
3.2.1.51
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
NaBH4-reduced oligosaccharide, very slow hydrolysis
208702
Pomacea canaliculata
Gal-beta-(1-4)-Glc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
very slow hydrolysis, NaBH4-reduced oligosaccharide
208702
Pomacea canaliculata
Gal-beta-(1-4)-Glc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O
-
208702
Pomacea canaliculata
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
3.2.1.51
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O
subscript OT is used to indicate NaBH4-reduced oligosaccharide
208702
Pomacea canaliculata
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
-
208702
Pomacea canaliculata
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
tetramer
4 * 60000, SDS-PAGE
Pomacea canaliculata
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
2.5
-
two pH-optima
Pomacea canaliculata
3.2.1.51
5
-
two pH-optima
Pomacea canaliculata