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Literature summary extracted from
- Purification and characterization of an alpha-L-fucosidase from Pomacea canaliculata (1993), Arch. Biochem. Biophys., 302, 152-160.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.51 | analysis | studies of structures and functions of glycoconjugates and oligosaccharides due to unique substrate specificity | Pomacea canaliculata |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.51 | 0.45 | - |
4-nitrophenyl-alpha-L-fucopyranoside | - |
Pomacea canaliculata |  |
| 3.2.1.51 | 1.18 | - |
2'-fucosyllactitol | - |
Pomacea canaliculata | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.51 | 60000 | - |
4 * 60000, SDS-PAGE | Pomacea canaliculata |
| 3.2.1.51 | 260000 | - |
gel filtration | Pomacea canaliculata |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.51 | 2'-fucosyllactitol + H2O | Pomacea canaliculata | - |
alpha-L-fucose + lactitol | - |
? | |
| 3.2.1.51 | Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O | Pomacea canaliculata | lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide | Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose | - |
? | |
| 3.2.1.51 | Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | Pomacea canaliculata | lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide | Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose | - |
? | |
| 3.2.1.51 | Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | Pomacea canaliculata | hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage | Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose | - |
? | |
| 3.2.1.51 | Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O | Pomacea canaliculata | lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
| 3.2.1.51 | Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O | Pomacea canaliculata | very slow hydrolysis, NaBH4-reduced oligosaccharide | Gal-beta-(1-4)-Glc + alpha-L-fucose | - |
? | |
| 3.2.1.51 | [Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O | Pomacea canaliculata | - |
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.51 | Pomacea canaliculata | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.51 | to homogeneity, chromatography steps | Pomacea canaliculata |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.51 | hepatopancreas | - |
Pomacea canaliculata | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.51 | 5.3 | - |
- |
Pomacea canaliculata |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.51 | 2'-fucosyllactitol + H2O | - |
Pomacea canaliculata | alpha-L-fucose + lactitol | - |
? | |
| 3.2.1.51 | 4-nitrophenyl-alpha-L-fucopyranoside + H2O | - |
Pomacea canaliculata | 4-nitrophenol + alpha-L-fucose | - |
? | |
| 3.2.1.51 | Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O | lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide | Pomacea canaliculata | Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose | - |
? | |
| 3.2.1.51 | Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide | Pomacea canaliculata | Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose | - |
? | |
| 3.2.1.51 | Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | NaBH4-reduced oligosaccharide, hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage | Pomacea canaliculata | Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose | - |
? | |
| 3.2.1.51 | Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage | Pomacea canaliculata | Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose | - |
? | |
| 3.2.1.51 | Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O | lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide | Pomacea canaliculata | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
| 3.2.1.51 | Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O | lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide | Pomacea canaliculata | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
| 3.2.1.51 | Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O | NaBH4-reduced oligosaccharide, very slow hydrolysis | Pomacea canaliculata | Gal-beta-(1-4)-Glc + alpha-L-fucose | - |
? | |
| 3.2.1.51 | Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O | very slow hydrolysis, NaBH4-reduced oligosaccharide | Pomacea canaliculata | Gal-beta-(1-4)-Glc + alpha-L-fucose | - |
? | |
| 3.2.1.51 | [Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O | - |
Pomacea canaliculata | [Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose | - |
? | |
| 3.2.1.51 | [Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O | subscript OT is used to indicate NaBH4-reduced oligosaccharide | Pomacea canaliculata | [Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.51 | tetramer | 4 * 60000, SDS-PAGE | Pomacea canaliculata |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.51 | 2.5 | - |
two pH-optima | Pomacea canaliculata |
| 3.2.1.51 | 5 | - |
two pH-optima | Pomacea canaliculata |
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