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Literature summary extracted from

  • Botti, M.G.; Taylor, M.G.; Botting, N.P.
    Studies on the mechanism of myrosinase. Investigation of the effect of glycosyl acceptors on enzymatic activity (1995), J. Biol. Chem., 270, 20530-20535.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.147 ascorbic acid activates Sinapis alba

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.147 delta-gluconolactone poor noncompetitive inhibitor Sinapis alba

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.147 0.42
-
 sinigrin with H2O or 6.25 M methanol as solvent Sinapis alba
3.2.1.147 0.54
-
 sinigrin with acetonitrile as solvent Sinapis alba
3.2.1.147 0.57
-
 sinigrin with 6.25 M ethanol as solvent Sinapis alba
3.2.1.147 0.57
-
 sinigrin with 12.5 M methanol as solvent Sinapis alba
3.2.1.147 0.87
-
 sinigrin with dioxane as solvent Sinapis alba
3.2.1.147 4
-
 p-nitrophenyl-beta-D-glucopyranoside with dioxane as solvent Sinapis alba
3.2.1.147 12
-
 p-nitrophenyl-beta-D-glucopyranoside with 12.5 M methanol as solvent Sinapis alba
3.2.1.147 17
-
 p-nitrophenyl-beta-D-glucopyranoside with acetonitrile as solvent Sinapis alba
3.2.1.147 30
-
 p-nitrophenyl-beta-D-glucopyranoside with 6.25 M methanol as solvent Sinapis alba
3.2.1.147 49
-
 p-nitrophenyl-beta-D-glucopyranoside with 0.2 M methanol as solvent Sinapis alba
3.2.1.147 51
-
 p-nitrophenyl-beta-D-glucopyranoside with 6.25 M ethanol as solvent Sinapis alba
3.2.1.147 61
-
 p-nitrophenyl-beta-D-glucopyranoside with water as solvent Sinapis alba

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Sinapis alba
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.147 seed
-
 Sinapis alba
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.147 additional information the enzyme does not catalyze a transglycosylation reaction either with alcohols or with other suitable glycosyl acceptors Sinapis alba ?
-
 ?
3.2.1.147 p-nitrophenyl beta-D-glucopyranoside + H2O
-
 Sinapis alba ?
-
 ?
3.2.1.147 sinigrin + H2O
-
 Sinapis alba D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?